ArticleBiophysical journal1998
Fusion pore conductance: experimental approaches and theoretical algorithms.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Initial size and dynamics of viral fusion pores are a function of the fusion protein mediating membrane fusion.Biology of the cell · 2008Article
- Cholesterol promotes hemifusion and pore widening in membrane fusion induced by influenza hemagglutinin.The Journal of general physiology · 2008Article
- Vesicle formation by self-assembly of membrane-bound matrix proteins into a fluidlike budding domain.The Journal of cell biology · 2007Article
- Determination of cell capacitance using the exact empirical solution of partial differential Y/partial differential Cm and its phase angle.Biophysical journal · 2004Article
- Shape bistability of a membrane neck: a toggle switch to control vesicle content release.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Effects of membrane potential and sphingolipid structures on fusion of Semliki Forest virus.Journal of virology · 2002Article
- Characterization of cholesterol-sphingomyelin domains and their dynamics in bilayer membranes.Biophysical journal · 2001Article
- Evolution of intermediates of influenza virus hemagglutinin-mediated fusion revealed by kinetic measurements of pore formation.Biophysical journal · 2001Article
- A point mutation in the transmembrane domain of the hemagglutinin of influenza virus stabilizes a hemifusion intermediate that can transit to fusion.Molecular biology of the cell · 2000Article
- Resolution of patch capacitance recordings and of fusion pore conductances in small vesicles.Biophysical journal · 2000Article
- Role of the cytoplasmic tail of ecotropic moloney murine leukemia virus Env protein in fusion pore formation.Journal of virology · 2000Article
- Hemifusion between cells expressing hemagglutinin of influenza virus and planar membranes can precede the formation of fusion pores that subsequently fully enlarge.Biophysical journal · 1999Article
- Tension of membranes expressing the hemagglutinin of influenza virus inhibits fusion.Biophysical journal · 1999Article
- A specific point mutant at position 1 of the influenza hemagglutinin fusion peptide displays a hemifusion phenotype.Molecular biology of the cell · 1999Article
- Amino acid sequence requirements of the transmembrane and cytoplasmic domains of influenza virus hemagglutinin for viable membrane fusion.Molecular biology of the cell · 1999Article
- Effects of spontaneous bilayer curvature on influenza virus-mediated fusion pores.The Journal of general physiology · 1998Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time-resolved admittance measurements provide the basis for studies showing that membrane fusion occurs through the formation and widening of an initially small pore, linking two previously separated aqueous compartments. Here we introduce modifications to this method that correct the cell-pipette (source) admittance for attenuation and phase shifts produced by electrophysiological equipment. Two new approaches for setting the right phase angle are discussed. The first uses the displacement of a patch-clamp amplifier C-slow potentiometer for the calculation of phase. This calculation is based on amplitudes of observed and expected (theoretical) changes in the source admittance. The second approach automates the original phase adjustment, the validity of which we prove analytically for certain conditions. The multiple sine wave approach is modified to allow the calculation of target cell membrane parameters and the conductance of the fusion pore. We also show how this technique can be extended for measurements of the resting potential of the first (voltage-clamped) membrane. We introduce an algorithm for calculation of fusion pore conductance despite a concurrent change in the resistance of the clamped membrane. The sensitivity of the capacitance restoration algorithm to phase shift errors is analyzed, and experimental data are used to demonstrate the results of this analysis. Finally, we show how the phase offset can be corrected "off-line" by restoring the shape of the capacitance increment.
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