ArticleBiophysical journal1998
Surface charge and lanthanum block of calcium current in bullfrog sympathetic neurons.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
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- Inhibition of astroglial hemichannels prevents synaptic transmission decline during spreading depression.Biological research · 2024Article
- Lysenin Channels as Sensors for Ions and Molecules.Sensors (Basel, Switzerland) · 2020Review
- Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Mechanisms of Spontaneous Electrical Activity in the Developing Cerebral Cortex-Mouse Subplate Zone.Cerebral cortex (New York, N.Y. : 1991) · 2019Article
- Article
- UTP - Gated Signaling Pathways of 5-HT Release from BON Cells as a Model of Human Enterochromaffin Cells.Frontiers in pharmacology · 2017Article
- Purinergic control of lysenin's transport and voltage-gating properties.Purinergic signalling · 2016Article
- Single channel measurements demonstrate the voltage dependence of permeation through N-type and L-type CaV channels.Channels (Austin, Tex.) · 2015Article
- Voltage control of Ca²⁺ permeation through N-type calcium (Ca(V)2.2) channels.The Journal of general physiology · 2014Article
- Ni2+ block of CaV3.1 (alpha1G) T-type calcium channels.The Journal of general physiology · 2008Article
- Y3+ block demonstrates an intracellular activation gate for the alpha1G T-type Ca2+ channel.The Journal of general physiology · 2004Article
- Ion trafficking through T-type Ca2+ channels: a way to look at channel gating position.The Journal of general physiology · 2004Article
- Molecular pharmacology of high voltage-activated calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- Overview of voltage-dependent calcium channels.Journal of bioenergetics and biomembranes · 1998Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The density of surface charge associated with the calcium channel pore was estimated from the effect of extracellular ionic strength on block by La3+. Currents carried by 2 mM Ba2+ were recorded from isolated frog sympathetic neurons by the whole-cell patch-clamp technique. In normal ionic strength (120 mM N-methyl-D-glucamine, NMG), La3+ blocked the current with high affinity (IC50 = 22 nM at 0 mV). La3+ block was relieved by strong depolarization in a time- and voltage-dependent manner. After unblocking, open channels reblocked rapidly at 0 mV, allowing estimation of association and dissociation rates for La3+: k(on) = (7.2 +/- 0.7) x 10(8) M(-1) s(-1), k(off) = 10.0 +/- 0.5 s(-1). To assess surface charge effects, La3+ block was also measured in low ionic strength (12.5 mM NMG) and high ionic strength (250 mM NMG). La3+ block was higher affinity and faster by two- to threefold in 12.5 mM NMG, with little effect of 250 mM NMG. The data could be described by Gouy-Chapman theory with a surface charge density of approximately 1 e-/3000-4000 A2. These results indicate that there is a small but detectable surface charge associated with the pore of voltage-dependent calcium channels.
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