ArticleIn vitro cellular & developmental biology. Animal1998
Simulated microgravity conditions enhance differentiation of cultured PC12 cells towards the neuroendocrine phenotype.
Article in In vitro cellular & developmental biology. Animal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Novel, low-cost bioreactor forFrontiers in bioengineering and biotechnology · 2025Article
- Bioreactor Technologies for Enhanced Organoid Culture.International journal of molecular sciences · 2023Review
- Differentiation of human induced pluripotent stem cells into functional lung alveolar epithelial cells in 3D dynamic culture.Frontiers in bioengineering and biotechnology · 2023Article
- The Fight against Cancer by Microgravity: The Multicellular Spheroid as a Metastasis Model.International journal of molecular sciences · 2022Review
- Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications.Bioengineering (Basel, Switzerland) · 2021Review
- The effects of microgravity on differentiation and cell growth in stem cells and cancer stem cells.Stem cells translational medicine · 2020Article
- Magnetic levitational bioassembly of 3D tissue construct in space.Science advances · 2020Article
- An Air Bubble-Isolating Rotating Wall Vessel Bioreactor for Improved Spheroid/Organoid Formation.Tissue engineering. Part C, Methods · 2019Article
- Simulated microgravity increases polyploid giant cancer cells and nuclear localization of YAP.Scientific reports · 2019Article
- Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.Infection and immunity · 2018Review
- 3D tissue-like assemblies: A novel approach to investigate virus-cell interactions.Methods (San Diego, Calif.) · 2015Article
- Hypergravity stimulation enhances PC12 neuron-like cell differentiation.BioMed research international · 2015Article
- Analysis of embryoid bodies derived from human induced pluripotent stem cells as a means to assess pluripotency.Stem cells international · 2012Article
- Closing the phenotypic gap between transformed neuronal cell lines in culture and untransformed neurons.Journal of neuroscience methods · 2008Article
- The role of connexins in the differentiation of NT2 cells in Sertoli-NT2 cell tissue constructs grown in the rotating wall bioreactor.Experimental brain research · 2006Article
- Effect of simulated microgravity on oxidation-sensitive gene expression in PC12 cells.Advances in space research : the official journal of the Committee on Space Research (COSPAR) · 2006Article
- Low-Shear modeled microgravity alters the Salmonella enterica serovar typhimurium stress response in an RpoS-independent manner.Applied and environmental microbiology · 2002Article
- Characterization of aggregation and protein expression of bovine corneal endothelial cells as microcarrier cultures in a rotating-wall vessel.Cytotechnology · 2000Article
- Transient maintenance in bioreactor improves health of neuronal cells.In vitro cellular & developmental biology. AnimalArticle
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We are studying microenvironmental cues which contribute to neuroendocrine organ assembly and tissue-specific differentiation. As our in vitro model, we cultured rat adrenal medullary PC12 pheochromocytoma cells in a novel cell culture system, the NASA rotating wall vessel (RWV) bioreactors. This "simulated microgravity" environment in RWV bioreactors, characterized by randomizing gravitational vectors and minimizing shear stress, has been shown to favor macroscopic tissue assembly and to induce tissue-specific differentiation. We hypothesized that the unique culture conditions in the RWV bioreactors might enhance the in vitro formation of neuroendocrine organoids. To test our hypothesis, we evaluated the expression of several markers of neuroendocrine differentiation in cultures of PC12 cells maintained for up to 20 d in the slow turning lateral vessel (STLV) type RWV. PC12 cell differentiation was assessed by morphological, immunological, biochemical and molecular techniques. PC12 cells, cultured under "simulated microgravity" conditions, formed macroscopic, tissue-like organoids several millimeters in diameter. Concomitantly, the expression of phenylethanolamine-N-methyl transferase (PNMT), but not of other catecholamine synthesizing enzymes, was enhanced. Increased PNMT expression, as verified on both the gene and protein level, was accompanied by an increase in the specific activity of the enzyme. Furthermore, after 20 d in culture in the STLV, we observed altered patterns of protein tyrosine phosphorylation and prolonged activation of c-fos, a member of the AP-1 nuclear transcription factor complex. We conclude that culture conditions in the RWV appear to selectively activate signal transduction pathways leading to enhanced neuroendocrine differentiation of PC12 cells.
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