ArticleGenes & development1998
Modulation of telomerase activity by telomere DNA-binding proteins in Oxytricha.
Article in Genes & development, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Evolving Linear Chromosomes and Telomeres: A C-Strand-Centric View.Trends in biochemical sciences · 2018Review
- Progress in Human and Tetrahymena Telomerase Structure Determination.Annual review of biophysics · 2017Review
- Multiple facets of TPP1 in telomere maintenance.Biochimica et biophysica acta · 2014Review
- Practical strategies for the evaluation of high-affinity protein/nucleic acid interactions.Journal of nucleic acids investigation · 2013Article
- Article
- Telomerase and telomere-associated proteins: structural insights into mechanism and evolution.Structure (London, England : 1993) · 2012Review
- Pot1 and telomere maintenance.FEBS letters · 2010Review
- Yeast telomerase subunit Est1p has guanine quadruplex-promoting activity that is required for telomere elongation.Nature structural & molecular biology · 2010Article
- Predictable suppression of gene expression by 5'-UTR-based RNA quadruplexes.Nucleic acids research · 2009Article
- Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p.Nucleic acids research · 2009Article
- Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres.Nucleic acids research · 2007Article
- Structural reorganization and the cooperative binding of single-stranded telomere DNA in Sterkiella nova.The Journal of biological chemistry · 2006Article
- DNA binding affinity and sequence permutation preference of the telomere protein from Euplotes crassus.Biochemistry · 2006Article
- Thermodynamic characterization of binding Oxytricha nova single strand telomere DNA with the alpha protein N-terminal domain.Journal of molecular biology · 2006Article
- Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Binding linkage in a telomere DNA-protein complex at the ends of Oxytricha nova chromosomes.Journal of molecular biology · 2005Article
- Distinct requirements for Pot1 in limiting telomere length and maintaining chromosome stability.Molecular and cellular biology · 2005Article
- Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro.Molecular and cellular biology · 2005Article
- Nucleotide shuffling and ssDNA recognition in Oxytricha nova telomere end-binding protein complexes.The EMBO journal · 2003Article
- Nucleic acid recognition by OB-fold proteins.Annual review of biophysics and biomolecular structure · 2003Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Telomere proteins protect the chromosomal terminus from nucleolytic degradation and end-to-end fusion, and they may contribute to telomere length control and the regulation of telomerase. The current studies investigate the effect of Oxytricha single-stranded telomere DNA-binding protein subunits alpha and beta on telomerase elongation of telomeric DNA. A native agarose gel system was used to evaluate telomere DNA-binding protein complex composition, and the ability of telomerase to use these complexes as substrates was characterized. Efficient elongation occurred in the presence of the alpha subunit. Moreover, the alpha-DNA cross-linked complex was a substrate for telomerase. At higher alpha concentrations, two alpha subunits bound to the 16-nucleotide single-stranded DNA substrate and rendered it inaccessible to telomerase. The formation of this alpha . DNA . alpha complex may contribute to regulation of telomere length. The alpha . beta . DNA ternary complex was not a substrate for telomerase. Even when telomerase was prebound to telomeric DNA, the addition of alpha and beta inhibited elongation, suggesting that these telomere protein subunits have a greater affinity for the DNA and are able to displace telomerase. In addition, the ternary complex was not a substrate for terminal deoxynucleotidyltransferase. We conclude that the telomere protein inhibits telomerase by rendering the telomeric DNA inaccessible, thereby helping to maintain telomere length.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.