ArticleThe Journal of cell biology1998
Evidence for a role of CLIP-170 in the establishment of metaphase chromosome alignment.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 146 citations in OpenAlex.
- Microtubule organizing centers regulate spindle positioning in mouse oocytes.Developmental cell · 2022Article
- CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments.The Journal of cell biology · 2020Article
- The kinetochore-microtubule interface at a glance.Journal of cell science · 2018Review
- Cytoplasmic Linker Protein CLIP170 Negatively Regulates TLR4 Signaling by Targeting the TLR Adaptor Protein TIRAP.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Spindle asymmetry drives non-Mendelian chromosome segregation.Science (New York, N.Y.) · 2017Article
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- Chromosome misalignments induce spindle-positioning defects.EMBO reports · 2016Article
- Microtubule plus end-associated CLIP-170 initiates HSV-1 retrograde transport in primary human cells.The Journal of cell biology · 2015Article
- A dynein independent role of Tctex-1 at the kinetochore.Cell cycle (Georgetown, Tex.) · 2015Article
- Novel piperazine-based compounds inhibit microtubule dynamics and sensitize colon cancer cells to tumor necrosis factor-induced apoptosis.The Journal of biological chemistry · 2014Article
- Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.The Journal of cell biology · 2013Article
- Microtubule plus-ends within a mitotic cell are 'moving platforms' with anchoring, signalling and force-coupling roles.Open biology · 2012Review
- Phosphorylation of CLIP-170 by Plk1 and CK2 promotes timely formation of kinetochore-microtubule attachments.The EMBO journal · 2010Article
- Article
- Phosphorylation controls autoinhibition of cytoplasmic linker protein-170.Molecular biology of the cell · 2010Article
- Finding the middle ground: how kinetochores power chromosome congression.Cellular and molecular life sciences : CMLS · 2010Review
- Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.PloS one · 2010Article
- Probing interactions between CLIP-170, EB1, and microtubules.Journal of molecular biology · 2010Article
- Effect of GFP tags on the localization of EB1 and EB1 fragments in vivo.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Stu1 inversely regulates kinetochore capture and spindle stability.Genes & development · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
CLIPs (cytoplasmic linker proteins) are a class of proteins believed to mediate the initial, static interaction of organelles with microtubules. CLIP-170, the CLIP best characterized to date, is required for in vitro binding of endocytic transport vesicles to microtubules. We report here that CLIP-170 transiently associates with prometaphase chromosome kinetochores and codistributes with dynein and dynactin at kinetochores, but not polar regions, during mitosis. Like dynein and dynactin, a fraction of the total CLIP-170 pool can be detected on kinetochores of unattached chromosomes but not on those that have become aligned at the metaphase plate. The COOH-terminal domain of CLIP-170, when transiently overexpressed, localizes to kinetochores and causes endogenous full-length CLIP-170 to be lost from the kinetochores, resulting in a delay in prometaphase. Overexpression of the dynactin subunit, dynamitin, strongly reduces the amount of CLIP-170 at kinetochores suggesting that CLIP-170 targeting may involve the dynein/dynactin complex. Thus, CLIP-170 may be a linker for cargo in mitosis as well as interphase. However, dynein and dynactin staining at kinetochores are unaffected by this treatment and further overexpression studies indicate that neither CLIP-170 nor dynein and dynactin are required for the formation of kinetochore fibers. Nevertheless, these results strongly suggest that CLIP-170 contributes in some way to kinetochore function in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.