ArticleMolecular and cellular biology1998
The second-largest subunit of the mouse DNA polymerase alpha-primase complex facilitates both production and nuclear translocation of the catalytic subunit of DNA polymerase alpha.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Karyopherin-mediated nucleocytoplasmic transport.Nature reviews. Molecular cell biology · 2022Review
- Pol α-primase dependent nuclear localization of the mammalian CST complex.Communications biology · 2021Article
- Quaternary structural diversity in eukaryotic DNA polymerases: monomeric to multimeric form.Current genetics · 2020Review
- Identification of FERM domain-containing protein 5 as a novel target of β-catenin/TCF7L2 complex.Cancer science · 2017Article
- The C-terminal domain of the DNA polymerase catalytic subunit regulates the primase and polymerase activities of the human DNA polymerase α-primase complex.The Journal of biological chemistry · 2014Article
- Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase.The Journal of biological chemistry · 2012Article
- Stimulation of BK virus DNA replication by NFI family transcription factors.Journal of virology · 2012Article
- Structure of a DNA polymerase alpha-primase domain that docks on the SV40 helicase and activates the viral primosome.The Journal of biological chemistry · 2010Article
- Aberrant DNA polymerase alpha is excluded from the nucleus by defective import and degradation in the nucleus.The Journal of biological chemistry · 2009Article
- Human Mcm10 regulates the catalytic subunit of DNA polymerase-alpha and prevents DNA damage during replication.Molecular biology of the cell · 2007Article
- Role of the p68 subunit of human DNA polymerase alpha-primase in simian virus 40 DNA replication.Molecular and cellular biology · 2002Article
- Cloning and characterization of the 5'-upstream sequence governing the cell cycle-dependent transcription of mouse DNA polymerase alpha 68 kDa subunit gene.Nucleic acids research · 2000Article
- Molecular architecture of the mouse DNA polymerase alpha-primase complex.Molecular and cellular biology · 1999Article
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Authors and funding
7 authors.
Funding
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Abstract
DNA polymerase alpha-primase is a replication enzyme necessary for DNA replication in all eukaryotes examined so far. Mouse DNA polymerase alpha is made up of four subunits, the largest of which is the catalytic subunit with a molecular mass of 180 kDa (p180). This subunit exists as a tight complex with the second-largest subunit (p68), whose physiological role has remained unclear up until now. We set out to characterize these subunits individually or in combination by using a cDNA expression system in cultured mammalian cells. Coexpression of p68 markedly increased the protein level of p180, with the result that ectopically generated DNA polymerase activity was dramatically increased. Immunofluorescence analysis showed that while either singly expressed p180 or p68 was localized in the cytoplasm, cotransfection of both subunits resulted in colocalization in the nucleus. We identified a putative nuclear localization signal for p180 (residues 1419 to 1437) and found that interaction with p68 is essential for p180 to translocate into the nucleus. These results indicate that association of p180 with p68 is important for both protein synthesis of p180 and translocation into the nucleus, implying that p68 plays a pivotal role in the newly synthesized DNA polymerase alpha complex.
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