ArticleMolecular and cellular biology1998
Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 136 citations in OpenAlex.
- The double life of mammalian DNA replication origins.Genes & development · 2025Review
- The chromatin environment shapes DNA replication origin organization and defines origin classes.Genome research · 2015Article
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Replication of Epstein-Barr viral DNA.Cold Spring Harbor perspectives in biology · 2013Review
- Initiation of DNA Replication in the Human Genome.Hereditary genetics : current research · 2012Article
- Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2.Molecular and cellular biology · 2011Article
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Isolation of a Drosophila amplification origin developmentally activated by transcription.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Overreplication of short DNA regions during S phase in human cells.Genes & development · 2008Article
- Discrete functional elements required for initiation activity of the Chinese hamster dihydrofolate reductase origin beta at ectopic chromosomal sites.Experimental cell research · 2007Article
- Mapping of DNA replication origins to noncoding genes of the X-inactivation center.Molecular and cellular biology · 2006Article
- DNA bending in the replication zone of the C3 DNA puff amplicon of Rhynchosciara americana (Diptera: Sciaridae).Molecular biology reports · 2006Article
- Essential elements of a licensed, mammalian plasmid origin of DNA synthesis.Molecular and cellular biology · 2006Article
- The Chinese hamster dihydrofolate reductase replication origin decision point follows activation of transcription and suppresses initiation of replication within transcription units.Molecular and cellular biology · 2006Article
- Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.Genes & development · 2005Article
- Initiation of DNA replication at the human beta-globin 3' enhancer.Nucleic acids research · 2005Article
- Coordination of replication and transcription along a Drosophila chromosome.Genes & development · 2004Article
- In search of the holy replicator.Nature reviews. Molecular cell biology · 2004Review
- Role for Cdk1 (Cdc2)/cyclin A in preventing the mammalian origin recognition complex's largest subunit (Orc1) from binding to chromatin during mitosis.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian replication origins appear paradoxical. While some studies conclude that initiation occurs bidirectionally from specific loci, others conclude that initiation occurs at many sites distributed throughout large DNA regions. To clarify this issue, the relative number of early replication bubbles was determined at 26 sites in a 110-kb locus containing the dihydrofolate reductase (DHFR)-encoding gene in CHO cells; 19 sites were located within an 11-kb sequence containing ori-beta. The ratio of approximately 0.8-kb nascent DNA strands to nonreplicated DNA at each site was quantified by competitive PCR. Nascent DNA was defined either as DNA that was labeled by incorporation of bromodeoxyuridine in vivo or as RNA-primed DNA that was resistant to lambda-exonuclease. Two primary initiation sites were identified within the 12-kb region, where two-dimensional gel electrophoresis previously detected a high frequency of replication bubbles. A sharp peak of nascent DNA occurred at the ori-beta origin of bidirectional replication where initiation events were 12 times more frequent than at distal sequences. A second peak occurred 5 kb downstream at a previously unrecognized origin (ori-beta'). Thus, the DHFR gene initiation zone contains at least three primary initiation sites (ori-beta, ori-beta', and ori-gamma), suggesting that initiation zones in mammals, like those in fission yeast, consist of multiple replication origins.
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