ArticleMolecular and cellular biology1998
Cooperation among multiple transcription factors is required for access to minimal T-cell receptor alpha-enhancer chromatin in vivo.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- The insulator EACBE regulates V(D)J recombination of Tcrd gene by modulating chromatin organization.Frontiers in immunology · 2025Article
- Regulation of T-cell Receptor Gene Expression by Three-Dimensional Locus Conformation and Enhancer Function.International journal of molecular sciences · 2020Review
- Integrating binding and expression data to predict transcription factors combined function.BMC genomics · 2020Article
- T-cell receptor α enhancer is inactivated in αβ T lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- IL-7 coordinates proliferation, differentiation and Tcra recombination during thymocyte β-selection.Nature immunology · 2015Article
- Recent insights into the transcriptional control of the Tcra/Tcrd locus by distant enhancers during the development of T-lymphocytes.Transcription · 2015Review
- Article
- Tcra enhancer activation by inducible transcription factors downstream of pre-TCR signaling.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Ectopic T cell receptor-α locus control region activity in B cells is suppressed by direct linkage to two flanking genes at once.PloS one · 2010Article
- Transgenic Wnt/TCF pathway reporters: all you need is Lef?Oncogene · 2006Review
- Granulocyte-macrophage colony-stimulating factor enhancer activation requires cooperation between NFAT and AP-1 elements and is associated with extensive nucleosome reorganization.Molecular and cellular biology · 2004Article
- Transcriptional regulatory cascades in development: initial rates, not steady state, determine network kinetics.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Transgenic mice demonstrate novel promoter regions for tissue-specific expression of the urokinase receptor gene.The American journal of pathology · 2003Article
- T cell receptor (TCR) alpha/delta locus enhancer identity and position are critical for the assembly of TCR delta and alpha variable region genes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells.Genes & development · 2002Article
- Direct regulation of BCL-2 by FLI-1 is involved in the survival of FLI-1-transformed erythroblasts.The EMBO journal · 2002Article
- Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificity.Oncogene · 2001Review
- runt homology domain transcription factors (Runx, Cbfa, and AML) mediate repression of the bone sialoprotein promoter: evidence for promoter context-dependent activity of Cbfa proteins.Molecular and cellular biology · 2001Article
- TCRalpha enhancer activation occurs via a conformational change of a pre-assembled nucleo-protein complex.The EMBO journal · 2000Article
- Auto-inhibition of Ets-1 is counteracted by DNA binding cooperativity with core-binding factor alpha2.Molecular and cellular biology · 2000Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To understand the molecular basis for the dramatic functional synergy between transcription factors that bind to the minimal T-cell receptor alpha enhancer (Ealpha), we analyzed enhancer occupancy in thymocytes of transgenic mice in vivo by genomic footprinting. We found that the formation of a multiprotein complex on this enhancer in vivo results from the occupancy of previously identified sites for CREB/ATF, TCF/LEF, CBF/PEBP2, and Ets factors as well as from the occupancy of two new sites 5' of the CRE site, GC-I (which binds Sp1 in vitro) and GC-II. Significantly, although all sites are occupied on a wild-type Ealpha, all sites are unoccupied on versions of Ealpha with mutations in the TCF/LEF or Ets sites. Previous in vitro experiments demonstrated hierarchical enhancer occupancy with independent binding of LEF-1 and CREB. Our data indicate that the formation of a multiprotein complex on the enhancer in vivo is highly cooperative and that no single Ealpha binding factor can access chromatin in vivo to play a unique initiating role in its assembly. Rather, the simultaneous availability of multiple enhancer binding proteins is required for chromatin disruption and stable binding site occupancy as well as the activation of transcription and V(D)J recombination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.