ArticleMolecular and cellular biology1998
5' processing of tRNA precursors can Be modulated by the human La antigen phosphoprotein.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 117 citations in OpenAlex.
- A tRNA gene potential to activate interferon signaling involves selective termination and is suppressible by La protein/SSB.Nucleic acids research · 2025Article
- Key RNA-binding domains in the La protein establish tRNA modification levels in Trypanosoma brucei.Nucleic acids research · 2025Article
- Altered tRNA processing is linked to a distinct and unusual La protein in Tetrahymena thermophila.Nature communications · 2022Article
- Regulatory roles of tRNA-derived RNA fragments in human pathophysiology.Molecular therapy. Nucleic acids · 2021Review
- The nuclear and cytoplasmic activities of RNA polymerase III, and an evolving transcriptome for surveillance.Nucleic acids research · 2021Article
- The many faces of RNA-based RNase P, an RNA-world relic.Trends in biochemical sciences · 2021Review
- Review
- La proteins couple use of sequence-specific and non-specific binding modes to engage RNA substrates.RNA biology · 2021Review
- The La-related proteins: structures and interactions of a versatile superfamily of RNA-binding proteins.RNA biology · 2021Review
- Role of the RNA-binding protein La in cancer pathobiology.RNA biology · 2021Review
- An interdomain bridge influences RNA binding of the human La protein.The Journal of biological chemistry · 2019Article
- Noncoding RNA Surveillance: The Ends Justify the Means.Chemical reviews · 2018Review
- SUMO Modification of the RNA-Binding Protein La Regulates Cell Proliferation and STAT3 Protein Stability.Molecular and cellular biology · 2018Article
- The RNA chaperone La promotes pre-tRNA maturation via indiscriminate binding of both native and misfolded targets.Nucleic acids research · 2017Article
- The La and related RNA-binding proteins (LARPs): structures, functions, and evolving perspectives.Wiley interdisciplinary reviews. RNA · 2017Review
- hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA.Nucleic acids research · 2016Article
- An La-related protein controls cell cycle arrest by nuclear retrograde transport of tRNAs during diapause formation in Artemia.BMC biology · 2016Article
- Integrity of SRP RNA is ensured by La and the nuclear RNA quality control machinery.Nucleic acids research · 2014Article
- Unusual noncanonical intron editing is important for tRNA splicing in Trypanosoma brucei.Molecular cell · 2013Article
- Regulation of cell death by transfer RNA.Antioxidants & redox signaling · 2013Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 4 countries.
Funding
Abstract
Eukaryotic precursor (pre)-tRNAs are processed at both ends prior to maturation. Pre-tRNAs and other nascent transcripts synthesized by RNA polymerase III are bound at their 3' ends at the sequence motif UUUOH [3' oligo(U)] by the La antigen, a conserved phosphoprotein whose role in RNA processing has been associated previously with 3'-end maturation only. We show that in addition to its role in tRNA 3'-end maturation, human La protein can also modulate 5' processing of pre-tRNAs. Both the La antigen's N-terminal RNA-binding domain and its C-terminal basic region are required for attenuation of pre-tRNA 5' processing. RNA binding and nuclease protection assays with a variety of pre-tRNA substrates and mutant La proteins indicate that 5' protection is a highly selective activity of La. This activity is dependent on 3' oligo(U) in the pre-tRNA for interaction with the N-terminal RNA binding domain of La and interaction of the C-terminal basic region of La with the 5' triphosphate end of nascent pre-tRNA. Phosphorylation of La is known to occur on serine 366, adjacent to the C-terminal basic region. We show that this modification interferes with the La antigen's ability to protect pre-tRNAiMet from 5' processing either by HeLa extract or purified RNase P but that it does not affect interaction with the 3' end of pre-tRNA. These findings provide the first evidence to indicate that tRNA 5'-end maturation may be regulated in eukaryotes. Implications of triphosphate recognition is discussed as is a role for La phosphoprotein in controlling transcriptional and posttranscriptional events in the biogenesis of polymerase III transcripts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.