ArticleGenetics1998
A screen for dynein synthetic lethals in Aspergillus nidulans identifies spindle assembly checkpoint genes and other genes involved in mitosis.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- Loss of theMicrobiology spectrum · 2025Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Location and functional analysis of the Aspergillus nidulans Aurora kinase confirm mitotic functions and suggest non-mitotic roles.Fungal genetics and biology : FG & B · 2017Article
- Spatial regulation of the spindle assembly checkpoint and anaphase-promoting complex in Aspergillus nidulans.Molecular microbiology · 2015Article
- Article
- Functional analysis of the Aspergillus nidulans kinome.PloS one · 2013Article
- Dynein and dynactin leverage their bivalent character to form a high-affinity interaction.PloS one · 2013Article
- Regulated inactivation of the spindle assembly checkpoint without functional mitotic spindles.The EMBO journal · 2011Article
- The RFX protein RfxA is an essential regulator of growth and morphogenesis in Penicillium marneffei.Eukaryotic cell · 2010Article
- Bub1 and Bub3 promote the conversion from monopolar to bipolar chromosome attachment independently of shugoshin.EMBO reports · 2009Article
- Zebrafish ale oko, an essential determinant of sensory neuron survival and the polarity of retinal radial glia, encodes the p50 subunit of dynactin.Development (Cambridge, England) · 2009Article
- Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans.Molecular biology of the cell · 2006Article
- Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans.Molecular biology of the cell · 2005Article
- SepBCTF4 is required for the formation of DNA-damage-induced UvsCRAD51 foci in Aspergillus nidulans.Genetics · 2005Article
- MesA, a novel fungal protein required for the stabilization of polarity axes in Aspergillus nidulans.Molecular biology of the cell · 2004Article
- Dual-Color imaging of nuclear division and mitotic spindle elongation in live cells of Aspergillus nidulans.Eukaryotic cell · 2004Article
- gamma-tubulin plays an essential role in the coordination of mitotic events.Molecular biology of the cell · 2004Article
- Roles of NUDE and NUDF proteins of Aspergillus nidulans: insights from intracellular localization and overexpression effects.Molecular biology of the cell · 2003Article
- Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.Genes & development · 2000Article
- The LIS1-related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein.The Journal of cell biology · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cytoplasmic dynein is a ubiquitously expressed microtubule motor involved in vesicle transport, mitosis, nuclear migration, and spindle orientation. In the filamentous fungus Aspergillus nidulans, inactivation of cytoplasmic dynein, although not lethal, severely impairs nuclear migration. The role of dynein in mitosis and vesicle transport in this organism is unclear. To investigate the complete range of dynein function in A. nidulans, we searched for synthetic lethal mutations that significantly reduced growth in the absence of dynein but had little effect on their own. We isolated 19 sld (synthetic lethality without dynein) mutations in nine different genes. Mutations in two genes exacerbate the nuclear migration defect seen in the absence of dynein. Mutations in six other genes, including sldA and sldB, show a strong synthetic lethal interaction with a mutation in the mitotic kinesin bimC and, thus, are likely to play a role in mitosis. Mutations in sldA and sldB also confer hypersensitivity to the microtubule-destabilizing drug benomyl. sldA and sldB were cloned by complementation of their mutant phenotypes using an A. nidulans autonomously replicating vector. Sequencing revealed homology to the spindle assembly checkpoint genes BUB1 and BUB3 from Saccharomyces cerevisiae. Genetic interaction between dynein and spindle assembly checkpoint genes, as well as other mitotic genes, indicates that A. nidulans dynein plays a role in mitosis. We suggest a model for dynein motor action in A. nidulans that can explain dynein involvement in both mitosis and nuclear distribution.
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