ArticleThe EMBO journal1998
Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 62 citations in OpenAlex.
- Dynamics and evolutionary conservation of B complex protein recruitment during spliceosome activation.Nucleic acids research · 2025Article
- Dynamics and Evolutionary Conservation of B Complex Protein Recruitment During Spliceosome Activation.bioRxiv : the preprint server for biology · 2024Article
- Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.RNA (New York, N.Y.) · 2023Review
- Recruitment of a splicing factor to the nuclear lamina for its inactivation.Communications biology · 2022Article
- Smu1 and RED are required for activation of spliceosomal B complexes assembled on short introns.Nature communications · 2019Article
- An Allosteric Network for Spliceosome Activation Revealed by High-Throughput Suppressor Analysis inGenetics · 2019Article
- Basal-A Triple-Negative Breast Cancer Cells Selectively Rely on RNA Splicing for Survival.Molecular cancer therapeutics · 2017Article
- Structure of a pre-catalytic spliceosome.Nature · 2017Article
- Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor.BMC evolutionary biology · 2017Article
- Article
- Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome.RNA (New York, N.Y.) · 2016Article
- Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex.The EMBO journal · 2015Article
- Rearrangements within human spliceosomes captured after exon ligation.RNA (New York, N.Y.) · 2013Article
- Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.Molecular and cellular biology · 2013Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.PloS one · 2012Article
- Mechanism for Aar2p function as a U5 snRNP assembly factor.Genes & development · 2011Article
- Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function.Genetics · 2009Article
- Drosophila MFAP1 is required for pre-mRNA processing and G2/M progression.The Journal of biological chemistry · 2008Article
- Dynamic interactions of Ntr1-Ntr2 with Prp43 and with U5 govern the recruitment of Prp43 to mediate spliceosome disassembly.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The elaborate and energy-intensive spliceosome assembly pathway belies the seemingly simple chemistry of pre-mRNA splicing. Prp38p was previously identified as a protein required in vivo and in vitro for the first pre-mRNA cleavage reaction catalyzed by the spliceosome. Here we show that Prp38p is a unique component of the U4/U6.U5 tri-small nuclear ribonucleoprotein (snRNP) particle and is necessary for an essential step late in spliceosome maturation. Without Prp38p activity spliceosomes form, but arrest in a catalytically impaired state. Functional spliceosomes shed U4 snRNA before 5' splice-site cleavage. In contrast, Prp38p-defective spliceosomes retain U4 snRNA bound to its U6 snRNA base-pairing partner. Prp38p is the first tri-snRNP-specific protein shown to be dispensable for assembly, but required for conformational changes which lead to catalytic activation of the spliceosome.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.