ArticleThe EMBO journal1998
A single amino acid substitution in yeast eIF-5A results in mRNA stabilization.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed.
- eIF5A and polyamines restrict mRNA levels in response to ribosome stalls.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- PtoeIF5A1: A Pleiotropic Regulator of Development, PCD, and Salt Tolerance inPlants (Basel, Switzerland) · 2026Article
- PRKD1/EIF5A/β-catenin axis inhibits tumorigenesis and metastasis in non-small-cell lung cancer.Clinical and experimental medicine · 2026Article
- Article
- Eukaryotic Initiation Factor 5A2 Regulates Expression of Antiviral Genes.Journal of molecular biology · 2022Article
- Role of eIF5A in Mitochondrial Function.International journal of molecular sciences · 2022Review
- eIF5A is activated by virus infection or dsRNA and facilitates virus replication through modulation of interferon production.Frontiers in cellular and infection microbiology · 2022Article
- A translational program that suppresses metabolism to shield the genome.Nature communications · 2020Article
- Indications for a moonlighting function of translation factor aIF5A in the crenarchaeum Sulfolobus solfataricus.RNA biology · 2019Article
- Polyamines as Quality Control Metabolites Operating at the Post-Transcriptional Level.Plants (Basel, Switzerland) · 2019Review
- Insulin action on protein synthesis and its association with eIF5A expression and hypusination.Molecular biology reports · 2019Article
- PhDHS Is Involved in Chloroplast Development in Petunia.Frontiers in plant science · 2019Article
- Plant-Mediated RNAi for ControllingFrontiers in plant science · 2019Article
- Modification of translation factor aIF5A from Sulfolobus solfataricus.Extremophiles : life under extreme conditions · 2018Article
- Polyamines and Their Role in Virus Infection.Microbiology and molecular biology reviews : MMBR · 2017Review
- Hypusination of eIF5A as a Target for Antiviral Therapy.DNA and cell biology · 2017Article
- Evolution of the archaeal and mammalian information processing systems: towards an archaeal model for human disease.Cellular and molecular life sciences : CMLS · 2017Review
- Regulation of gene expression by translation factor eIF5A: Hypusine-modified eIF5A enhances nonsense-mediated mRNA decay in human cells.Translation (Austin, Tex.) · 2017Article
- Prognostic significance of the expression of nuclear eukaryotic translation initiation factor 5A2 in human melanoma.Oncology letters · 2016Article
- Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells.Scientific reports · 2016Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Most factors known to function in mRNA turnover are not essential for cell viability. To identify essential factors, approximately 4000 temperature-sensitive yeast strains were screened for an increase in the level of the unstable CYH2 pre-mRNA. At the non-permissive temperature, five mutants exhibited decreased decay rates of the CYH2 pre-mRNA and mRNA, and the STE2, URA5 and PAB1 mRNAs. Of these, the mutant ts1159 had the most extensive phenotype. Expression of the TIF51A gene (encoding eIF-5A) complemented the temperature-sensitive growth and mRNA decay phenotypes of ts1159. The tif51A allele was rescued from these cells and shown to encode a serine to proline change within a predicted alpha-helical segment of the protein. ts1159 also exhibited an approximately 30% decrease in protein synthesis at the restrictive temperature. Measurement of amino acid incorporation in wild-type cells incubated with increasing amounts of cycloheximide demonstrated that a decrease in protein synthesis of this magnitude could not account for the full extent of the mRNA decay defects observed in ts1159. Interestingly, the ts1159 cells accumulated uncapped mRNAs at the non-permissive temperature. These results suggest that eIF-5A plays a role in mRNA turnover, perhaps acting downstream of decapping.
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