ArticleGenome research1998
Sequencing multimegabase-template DNA with BigDye terminator chemistry.
Article in Genome research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- No Evidence of Pritelivir Resistance Among Herpes Simplex Virus Type 2 Isolates After 4 Weeks of Daily Therapy.The Journal of infectious diseases · 2016Trial
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- Description of Flavobacterium agricola sp. nov., an auxin producing bacterium isolated from paddy field.Antonie van Leeuwenhoek · 2023Article
- Utility of targeted next generation sequencing for inborn errors of immunity at a tertiary care centre in North India.Scientific reports · 2022Article
- Pusillimonas faecipullorum sp. nov., isolated from the poultry manure.Archives of microbiology · 2022Article
- Mutation in BEIIb mitigates the negative effect of the mutation in ISA1 on grain filling and amyloplast formation in rice.Plant molecular biology · 2022Article
- Zeimonas arvi gen. nov., sp. nov., of the family Burkholderiaceae, harboring biphenyl- and phenolic acid-metabolizing genes, isolated from a long-term ecological research field.Antonie van Leeuwenhoek · 2021Article
- Bioremediation of palm oil mill effluent (POME) using indigenous Meyerozyma guilliermondii.Environmental science and pollution research international · 2019Article
- Mycoplasmas under experimental antimicrobial selection: The unpredicted contribution of horizontal chromosomal transfer.PLoS genetics · 2019Article
- Global Changes in Mycoplasma gallisepticum Phase-Variable Lipoprotein Gene vlhA Expression during In Vivo Infection of the Natural Chicken Host.Infection and immunity · 2016Article
- A CYP26B1 polymorphism enhances retinoic acid catabolism and may aggravate atherosclerosis.Molecular medicine (Cambridge, Mass.) · 2012Article
- Rheonix CARD(®) Technology: An Innovative and Fully Automated Molecular Diagnostic Device.Point of care · 2012Article
- Virulence factors encoded by Legionella longbeachae identified on the basis of the genome sequence analysis of clinical isolate D-4968.Journal of bacteriology · 2010Article
- An asymmetric model for Na+-translocating glutaconyl-CoA decarboxylases.The Journal of biological chemistry · 2009Article
- Occurrence, plasticity, and evolution of the vpma gene family, a genetic system devoted to high-frequency surface variation in Mycoplasma agalactiae.Journal of bacteriology · 2009Article
- Article
- Characterization of bacterial operons consisting of two tubulins and a kinesin-like gene by the novel Two-Step Gene Walking method.Nucleic acids research · 2007Article
- Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Identification of a virulence-associated determinant, dihydrolipoamide dehydrogenase (lpd), in Mycoplasma gallisepticum through in vivo screening of transposon mutants.Infection and immunity · 2006Article
- Bacteriophage MAV1 is not associated with virulence of Mycoplasma arthritidis.Infection and immunity · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Using the recently introduced BigDye terminators, large-template DNA can be directly sequenced with custom primers on automated instruments. Cycle sequencing conditions are presented to sequence DNA samples isolated from a number of microbial genomes including 750-kb Ureaplasma urealyticum, 1.2-Mb Mycoplasma fermentans, 2.3-Mb Streptococcus pneumoniae, and 4.6-Mb Escherichia coli. Average read lengths of >700 bp from unique primer annealing sites are often sufficient to fill final gaps in microbial genome sequencing projects without additional manipulations of template DNA. The technique can also be applied to sequence-targeted regions, thereby bypassing tedious subcloning steps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.