ArticleRNA (New York, N.Y.)1998
Group I-like ribozymes with a novel core organization perform obligate sequential hydrolytic cleavages at two processing sites.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Animal-associated jumbo phages as widespread and active modulators of gut microbiome ecology and metabolism.Science advances · 2026Article
- A prevalent huge phage clade in human and animal gut microbiomes.bioRxiv : the preprint server for biology · 2025Article
- Using Rosetta for RNA homology modeling.Methods in enzymology · 2019Article
- Review
- Speciation of a group I intron into a lariat capping ribozyme.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Molecular characterization of a new member of the lariat capping twin-ribozyme introns.Mobile DNA · 2014Article
- Predicting coaxial helical stacking in RNA junctions.Nucleic acids research · 2012Article
- A conformational switch in the DiGIR1 ribozyme involved in release and folding of the downstream I-DirI mRNA.RNA (New York, N.Y.) · 2009Article
- Molecular modelling of the GIR1 branching ribozyme gives new insight into evolution of structurally related ribozymes.The EMBO journal · 2008Article
- Searching genomes for ribozymes and riboswitches.Genome biology · 2007Review
- Short-term sequence evolution and vertical inheritance of the Naegleria twin-ribozyme group I intron.BMC evolutionary biology · 2006Article
- Topology of three-way junctions in folded RNAs.RNA (New York, N.Y.) · 2006Article
- Site-specific reverse splicing of a HEG-containing group I intron in ribosomal RNA.Nucleic acids research · 2005Article
- The ability to form full-length intron RNA circles is a general property of nuclear group I introns.RNA (New York, N.Y.) · 2003Article
- Flanking sequences with an essential role in hydrolysis of a self-cleaving group I-like ribozyme.Nucleic acids research · 2000Article
- Expression of the Naegleria intron endonuclease is dependent on a functional group I self-cleaving ribozyme.RNA (New York, N.Y.) · 2000Article
- In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron.The EMBO journal · 1999Article
- In vitro selection of the Naegleria GIR1 ribozyme identifies three base changes that dramatically improve activity.RNA (New York, N.Y.) · 1998Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new category of self-splicing group I introns with conserved structural organization and function is found among the eukaryotic microorganisms Didymium and Naegleria. These complex rDNA introns contain two distinct ribozymes with different functions: a regular group I splicing-ribozyme and a small internal group I-like ribozyme (GIR1), probably involved in protein expression. GIR1 was found to cleave at two internal sites in an obligate sequential order. Both sites are located 3' of the catalytic core. GIR1-catalyzed transesterification reactions could not be detected. We have compared all available GIR1 sequences and propose a common RNA secondary structure resembling that of group I splicing-ribozymes, but with some important differences. The GIR1s lack most peripheral sequence components, as well as a P1 segment, and, at approximately 160-190 nt, they are the smallest functional group I ribozymes known from nature. All GIR1s were found to contain a novel 6-bp pseudoknot (P15) within their catalytic core region. Experimental support of the proposed structure was obtained from the Didymium GIR1 by RNA structure probing and site-directed mutagenesis. Three-dimensional modeling indicates a compactly folded ribozyme with the functionally essential P15 exposed in the cleft between the two principal domains P3-P8 and P4-P6.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.