ArticleNucleic acids research1998
New positive/negative selectable markers for mammalian cells on the basis of Blasticidin deaminase-thymidine kinase fusions.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- An unexpected hydratase synthesizes the green light-absorbing pigment fucoxanthin.The Plant cell · 2023Article
- Article
- Green diatom mutants reveal an intricate biosynthetic pathway of fucoxanthin.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency.Nucleic acids research · 2021Article
- A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology.PloS one · 2019Article
- Streptomyces lividans blasticidin S deaminase and its application in engineering a blasticidin S-producing strain for ease of genetic manipulation.Applied and environmental microbiology · 2013Article
- Human embryonic stem cells: an in vitro model to study mechanisms controlling pluripotency in early mammalian development.Stem cell reviews · 2005Review
Corrections and comments
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Authors and funding
1 author.
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Abstract
Two positive and negative selectable markers were created for use in mammalian cells. They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by their ability to induce BlaS resistance and negatively on the induced sensitivity towards gancyclovir (GANC). Both constructs are also expressed in Escherichia coli and transfer BlaS resistance to this organism as well, making these markers very suitable for the construction of shuttle vectors.
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