ArticleNucleic acids research1998
High level transactivation by the ecdysone receptor complex at the core recognition motif.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Anatomy and evolution of a DNA replication origin.Chromosoma · 2021Article
- Structure and function of the alternatively spliced isoforms of the ecdysone receptor gene in the Chinese mitten crab, Eriocheir sinensis.Scientific reports · 2017Article
- Ligand-independent requirements of steroid receptors EcR and USP for cell survival.Cell death and differentiation · 2016Article
- CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions through the Ecdysone Receptor in Drosophila.PLoS biology · 2015Article
- Ash2 acts as an ecdysone receptor coactivator by stabilizing the histone methyltransferase Trr.Molecular biology of the cell · 2013Article
- Juvenile hormone action through a defined enhancer motif to modulate ecdysteroid-activation of natural core promoters.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology · 2012Article
- Selenoprotein P regulation by the glucocorticoid receptor.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2009Article
- Genomic mapping of binding regions for the Ecdysone receptor protein complex.Genome research · 2009Article
- Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain.Nucleic acids research · 2007Article
- Protein-DNA binding specificity predictions with structural models.Nucleic acids research · 2005Article
- Ligand control of interaction in vivo between ecdysteroid receptor and ultraspiracle ligand-binding domain.The Biochemical journal · 2004Article
- Structure of the heterodimeric ecdysone receptor DNA-binding complex.The EMBO journal · 2003Article
- Identification and in vivo characterization of the Epiphyas postvittana nucleopolyhedrovirus Ecdysteroid UDP-glucosyltransferase.Virus genes · 2001Article
- Activation of a delayed-early gene encoding MHR3 by the ecdysone receptor heterodimer EcR-B1-USP-1 but not by EcR-B1-USP-2.Molecular and cellular biology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ecdysteroid signaling in insects is mediated by the ecdysone receptor complex that is composed of a heterodimer of the ecdysone receptor and Ultraspiracle. The DNA binding specificity plays a critical role of defining the repertoire of target genes that respond to the hormone. We report here the determination of the preferred core recognition motif by a binding site selection procedure. The consensus sequence consists of a perfect palindrome of the heptameric half-site sequence GAGGTCA that is separated by a single A/T base pair. No binding polarity of the ecdysone receptor/Ultraspiracle heterodimer to the core recognition motif was observed. This core motif mediated the highest level of ligand-induced transactivation when compared to a series of synthetic ecdysone response elements and to the natural element of the Drosophila hsp27 gene. This is the first report of a palindromic sequence identified as the highest affinity DNA binding site for a heterodimeric nuclear hormone receptor complex. We further present evidence that the ligand of the ecdysone receptor preferentially drives Ultraspiracle from a homodimer into a heterodimer. This mechanism might contribute additionally to a tight control of target gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.