ArticleNucleic acids research1998
Structural requirements for DNA binding of GCM proteins.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
- New insights into the regulation of placental growth factor gene expression by the transcription factors GCM1 and DLX3 in human placenta.The Journal of biological chemistry · 2018Article
- Functional Conservation of the Glide/Gcm Regulatory Network Controlling Glia, Hemocyte, and Tendon Cell Differentiation in Drosophila.Genetics · 2016Article
- Generation of mice encoding a conditional null allele of Gcm2.Transgenic research · 2014Article
- Lineage specification in the fly nervous system and evolutionary implications.Cell cycle (Georgetown, Tex.) · 2013Review
- Identification and characterization of C106R, a novel mutation in the DNA-binding domain of GCMB, in a family with autosomal-dominant hypoparathyroidism.Clinical endocrinology · 2012Article
- Mutational analysis of GCMB, a parathyroid-specific transcription factor, in parathyroid adenoma of primary hyperparathyroidism.The Journal of endocrinology · 2011Article
- Mammalian Gcm genes induce Hes5 expression by active DNA demethylation and induce neural stem cells.Nature neuroscience · 2011Article
- Dominant-negative GCMB mutations cause an autosomal dominant form of hypoparathyroidism.The Journal of clinical endocrinology and metabolism · 2008Article
- bZIP-Type transcription factors CREB and OASIS bind and stimulate the promoter of the mammalian transcription factor GCMa/Gcm1 in trophoblast cells.Nucleic acids research · 2008Article
- Glial cell missing 1 regulates placental growth factor (PGF) gene transcription in human trophoblast.Biology of reproduction · 2008Article
- Exploring the recognition of quadruplex DNA by an engineered Cys2-His2 zinc finger protein.Biochemistry · 2006Article
- Stimulation of GCMa transcriptional activity by cyclic AMP/protein kinase A signaling is attributed to CBP-mediated acetylation of GCMa.Molecular and cellular biology · 2005Article
- Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis.Applied and environmental microbiology · 2005Article
- Impacts of a new transcription factor family: mammalian GCM proteins in health and disease.The Journal of cell biology · 2004Review
- 1H, 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein.Journal of biomolecular NMR · 2003Article
- Structure of the GCM domain-DNA complex: a DNA-binding domain with a novel fold and mode of target site recognition.The EMBO journal · 2003Article
- Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach.Nucleic acids research · 2002Article
- Glide2, a second glial promoting factor in Drosophila melanogaster.The EMBO journal · 2001Article
- Placental failure in mice lacking the mammalian homolog of glial cells missing, GCMa.Molecular and cellular biology · 2000Article
- Positive autoregulation of the glial promoting factor glide/gcm.The EMBO journal · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the GCM family of transcription factors contain a DNA binding domain unrelated to any other known DNA binding domain and bind to a DNA sequence motif not recognized by any other known transcription factor. Here we show that positions 2, 3, 6 and 7 of the 5'-ATGCGGGT-3' motif are particularly important for DNA binding and that methylation of several G residues on the upper strand, but not on the lower strand, interfered with binding of GCM proteins. No differences were detected between the DNA binding of Drosophila GCM and mammalian mGCMa. Alanine scan mutagenesis of the DNA binding domain of mGCMa identified the three conserved amino acids K74, C76 and C125 as being essential for DNA binding. Conserved cysteine residues were also found to be important for maintaining the overall integrity of the DNA binding domain and for mediating redox sensitivity of DNA binding. These cysteine residues are arranged in a symmetrical structure that bears no resemblance to other cysteine-containing structures, such as zinc fingers. In agreement with this, DNA binding of mGCMa was not dependent on zinc ions. Our results give insights into the exact nature of the GCM binding sites expected in target genes and point to a role for redox regulation in the function of GCM proteins.
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