ArticleNucleic acids research1998
Genomic footprinting of the yeast zinc finger protein Rme1p and its roles in repression of the meiotic activator IME1.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Choose Your Own Adventure: The Role of Histone Modifications in Yeast Cell Fate.Journal of molecular biology · 2017Review
- Long noncoding RNA-based chromatin control of germ cell differentiation: a yeast perspective.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Review
- Article
- Regulation of entry into gametogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Functional dissection of IME1 transcription using quantitative promoter-reporter screening.Genetics · 2010Article
- Transcription factors Mat2 and Znf2 operate cellular circuits orchestrating opposite- and same-sex mating in Cryptococcus neoformans.PLoS genetics · 2010Article
- Alpha3, a transposable element that promotes host sexual reproduction.Genes & development · 2010Article
- Genome reprogramming during sporulation.The International journal of developmental biology · 2009Review
- Yeast Ume6p repressor permits activator binding but restricts TBP binding at the HOP1 promoter.Nucleic acids research · 2003Article
- Genome-wide expression profile analysis reveals coordinately regulated genes associated with stepwise acquisition of azole resistance in Candida albicans clinical isolates.Antimicrobial agents and chemotherapy · 2003Article
- Repression and activation domains of RME1p structurally overlap, but differ in genetic requirements.Molecular biology of the cell · 2002Article
- Destabilization of nucleosomes by an unusual DNA conformation adopted by poly(dA) small middle dotpoly(dT) tracts in vivo.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The zinc finger protein Rme1p is a negative regulator of the meiotic activator IME1 in Saccharomyces cerevisiae . Prior studies have shown that Rme1p binds in vitro to a site near nt -2030 in the IME1 upstream region, but a genomic mutation in that site has little effect on repression of IME1 . To identify Rme1p binding sites in vivo , we have examined the binding of Rme1p to genomic sites through in vivo footprinting. We show that Rme1p binds to two sites in the IME1 upstream region, near nt -1950 and -2030. Mutations in both binding sites abolish repression of chromosomal IME1 by Rme1p, whereas a mutation in either single site causes partial derepression. Therefore, both Rme1p binding sites are essential for repression of IME1 . Prior studies have shown that repression by Rme1p depends upon RGR1 and SIN4 , which specify RNA polymerase II mediator subunits that are required for normal nucleosome density. We find that RGR1 and SIN4 are not simply required for Rme1p to bind to DNA in vivo . These results suggest that Rme1p functions directly as a repressor of IME1 and that Rgr1p and Sin4p are required for DNA-bound Rme1p to exert repression.
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Registered trials
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