ArticleNucleic acids research1998
Characterization of protein-binding to the spinach chloroplast psbA mRNA 5' untranslated region.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 56 citations in OpenAlex.
- The Chloroplast Ribonucleoprotein CP33B Quantitatively Binds thePlants (Basel, Switzerland) · 2020Article
- Processing of the 5'-UTR and existence of protein factors that regulate translation of tobacco chloroplast psbN mRNA.Plant molecular biology · 2014Article
- The atypical short-chain dehydrogenases HCF173 and HCF244 are jointly involved in translational initiation of the psbA mRNA of Arabidopsis.Plant physiology · 2012Article
- The role of heterologous chloroplast sequence elements in transgene integration and expression.Plant physiology · 2010Article
- Chloroplast ribonucleoprotein CP31A is required for editing and stability of specific chloroplast mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The nuclear-encoded factor HCF173 is involved in the initiation of translation of the psbA mRNA in Arabidopsis thaliana.The Plant cell · 2007Article
- Multiple elements required for translation of plastid atpB mRNA lacking the Shine-Dalgarno sequence.Nucleic acids research · 2004Article
- Expression and RNA binding properties of the chloroplast ribosomal protein S1 from Chlamydomonas reinhardtii.Plant molecular biology · 2003Article
- Chloroplast RNA-binding proteins.Current genetics · 2003Review
- An AU-box motif upstream of the SD sequence of light-dependent psbA transcripts confers mRNA instability in darkness in cyanobacteria.Nucleic acids research · 2001Article
- Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts.Plant physiology · 2001Article
- cis- and trans-Acting determinants for translation of psbD mRNA in Chlamydomonas reinhardtii.Molecular and cellular biology · 2000Article
- RNA-binding characteristics of the chloroplast S1-like ribosomal protein CS1.Nucleic acids research · 2000Article
- yellow-in-the-dark mutants of Chlamydomonas lack the CHLL subunit of light-independent protochlorophyllide reductase.The Plant cell · 2000Article
- Processing of the psbA 5' untranslated region in Chlamydomonas reinhardtii depends upon factors mediating ribosome association.The Journal of cell biology · 1998Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-binding proteins play a major role in regulating mRNA metabolism in chloroplasts. In this work we characterized two proteins, of 43 and 47 kDa, which bind to the spinach psbA mRNA 5' untranslated region (psbA encoding the D1 protein of photosystem II). The 43 kDa protein, which is present in the stroma and in membranes, co-sediments with a complex of 68S. It was purified, and the N-terminal sequence was determined. Upon homology search it was identified as the chloroplast homologue of the Escherichia coli ribosomal protein S1. The 47 kDa protein, which, in contrast with the 43 kDa protein, sediments with a small sedimentation coefficient, is only detected in the stromal fraction. It is soluble in an uncomplexed form. By deletion analysis, an element within the psbA mRNA 5' untranslated region was identified that is necessary but not sufficient for binding of stromal proteins. The 'central protein binding element' ranges from nucleotide -49 to -9 of the psbA mRNA 5' untranslated region. It comprises the Shine-Dalgarno-like GGAG motif and, 7 nucleotides upstream, an endonucleolytic cleavage site involved in psbA mRNA degradation in vitro . The mechanistic impacts of this region in relation to RNA-binding proteins are discussed.
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