ArticlePlant physiology1998
Tomato fructokinases exhibit differential expression and substrate regulation
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 98 citations in OpenAlex.
- Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7-2 in Wucai (Brassica campestris L.).BMC genomics · 2021Article
- Fruit setting rewires central metabolism via gibberellin cascades.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- An Overview of Sucrose Synthases in Plants.Frontiers in plant science · 2019Review
- Transcriptome analysis ofPlant methods · 2019Article
- Melatonin-Mediated Sugar Accumulation and Growth Inhibition in Apple Plants Involves Down-Regulation of Fructokinase 2 Expression and Activity.Frontiers in plant science · 2019Article
- A new insight into the evolution and functional divergence ofRoyal Society open science · 2018Article
- Plant Fructokinases: Evolutionary, Developmental, and Metabolic Aspects in Sink Tissues.Frontiers in plant science · 2018Review
- Increased activity of MdFRK2, a high-affinity fructokinase, leads to upregulation of sorbitol metabolism and downregulation of sucrose metabolism in apple leaves.Horticulture research · 2018Article
- Arabidopsis Fructokinases Are Important for Seed Oil Accumulation and Vascular Development.Frontiers in plant science · 2016Article
- Hexose kinases and their role in sugar-sensing and plant development.Frontiers in plant science · 2013Article
- Role of Vacuolar H+-inorganic pyrophosphatase in tomato fruit development.Journal of experimental botany · 2012Article
- LeFRK2 is required for phloem and xylem differentiation and the transport of both sugar and water.Planta · 2009Article
- Article
- Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plants.Planta · 2006Article
- Differential expression of two fructokinases in Oryza sativa seedlings grown under aerobic and anaerobic conditions.Journal of plant research · 2006Article
- Fructokinase and hexokinase from pollen grains of bell pepper (Capsicum annuum L.): possible role in pollen germination under conditions of high temperature and CO2 enrichment.Annals of botany · 2002Article
- Distinct physiological roles of fructokinase isozymes revealed by gene-specific suppression of Frk1 and Frk2 expression in tomato.Plant physiology · 2002Article
- Sugar sensing and signaling in plants.The Plant cell · 2002Review
- Overexpression of Arabidopsis hexokinase in tomato plants inhibits growth, reduces photosynthesis, and induces rapid senescence.The Plant cell · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two divergent genes encoding fructokinase, Frk1 and Frk2, have been previously shown to be expressed in tomato (Lycopersicon esculentum L.) and have now been further characterized with regard to their spatial expression and the enzymic properties of the encoded proteins. Frk1 and Frk2 mRNA levels were coordinately induced by exogenous sugar, indicating that both belong to the growing class of sugar-regulated genes. However, in situ hybridization indicated that Frk1 and Frk2 were expressed in a spatially distinct manner, with Frk2 mRNA primarily localized in cells of the fruit pericarp, which store starch, and Frk1 mRNA distributed ubiquitously in pericarp tissue. To evaluate the biochemical characteristics of the products of the Frk1 and Frk2 genes, each cDNA was expressed in a mutant yeast (Saccharomyces cerevisiae) line defective in hexose phosphorylation and unable to grow on glucose or fructose (Fru). Both Frk1 and Frk2 proteins expressed in yeast conferred the ability to grow on Fru and exhibited fructokinase activity in vitro. Although both Frk1 and Frk2 both utilized Fru as a substrate, only Frk2 activity was inhibited at high Fru concentrations. These results indicate that Frk2 can be distinguished from Frk1 by its sensitivity to substrate inhibition and by its temporal and spatial pattern of expression, which suggests that it plays a primary role in plant cells specialized for starch storage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.