ArticleJournal of clinical microbiology1998
Molecular epidemiological study of Aspergillus fumigatus in a bone marrow transplantation unit by PCR amplification of ribosomal intergenic spacer sequences.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Molecular Strain Typing of Clinical Isolates,Journal of clinical and diagnostic research : JCDR · 2017Article
- Phylogenetic analyses on the diversity of Aspergillus fumigatus sensu lato based on five orthologous loci.Mycopathologia · 2014Article
- Increased immunoglobulin G anti-Paracoccidioides brasiliensis serum antibody avidity as a predictor of favorable posttherapeutic evolution in paracoccidioidomycosis.Clinical and vaccine immunology : CVI · 2009Article
- Application of polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphism for detection and identification of dermatophytes from dermatological specimens.Indian journal of dermatology · 2008Article
- Genotyping of Trichophyton rubrum by analysis of ribosomal-DNA intergenic spacer regions.Mycopathologia · 2007Article
- Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus.Journal of clinical microbiology · 2007Article
- Identification to the species level and differentiation between strains of Aspergillus clinical isolates by automated repetitive-sequence-based PCR.Journal of clinical microbiology · 2004Article
- Aspergillus fumigatus variant with decreased susceptibility to multiple antifungals.Antimicrobial agents and chemotherapy · 2004Article
- Molecular typing study of the Microsporum canis strains isolated from an outbreak of tinea capitis in a school.Mycopathologia · 2004Article
- Restriction fragment length polymorphism analysis of ribosomal DNA intergenic regions is useful for differentiating strains of Trichophyton mentagrophytes.Journal of clinical microbiology · 2003Article
- Molecular epidemiology of Aspergillus fumigatus isolates recovered from water, air, and patients shows two clusters of genetically distinct strains.Journal of clinical microbiology · 2003Article
- Pulmonary infiltrates in immunosuppressed patients: analysis of a diagnostic protocol.Journal of clinical microbiology · 2002Article
- Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains.Journal of clinical microbiology · 2001Article
- Identification of Aspergillus fumigatus and related species by nested PCR targeting ribosomal DNA internal transcribed spacer regions.Journal of clinical microbiology · 2001Article
- Molecular epidemiology of Scedosporium apiospermum infection determined by PCR amplification of ribosomal intergenic spacer sequences in patients with chronic lung disease.Journal of clinical microbiology · 2001Article
- Molecular differentiation of seven Malassezia species.Journal of clinical microbiology · 2000Article
- Application of nucleic acid amplification in clinical microbiology.Molecular biotechnology · 1999Review
- Aspergillus fumigatus and aspergillosis.Clinical microbiology reviews · 1999Review
- Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a PCR-based method for the subspecific discrimination of Aspergillus fumigatus types by using two primers designed to amplify the intergenic spacer regions between ribosomal DNA transcription units. The method permitted the reproducible discrimination of 11 distinct DNA types among a total of 119 isolates of A. fumigatus collected from patients and from the environment of a bone marrow transplantation (BMT) unit over a three-year period. Ten DNA types of A. fumigatus were isolated from patients in the BMT unit; eight of these types were also found in the hospital environment, and six of these were present in the unit itself. Thirteen BMT patients developed infection with one of three DNA types some months after these had first been found in the environment of the unit. In other instances, the same DNA types of A. fumigatus were isolated from BMT patients that were later recovered from the environment of the unit. Several DNA types of A. fumigatus were found in the hospital environment over an 18-month period. Molecular typing of multiple isolates of A. fumigatus, obtained from postmortem tissue samples, showed that one patient was infected with a single DNA type, but two others had up to three different DNA types. Our findings suggest that A. fumigatus infection in BMT recipients may be nosocomial in origin and underline the need for careful environmental monitoring of units in which high-risk patients are housed.
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