ArticleJournal of virology1998
High-efficiency gene transfer into normal and adenosine deaminase-deficient T lymphocytes is mediated by transduction on recombinant fibronectin fragments.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 121 citations in OpenAlex.
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- Optimized peptide nanofibrils as efficient transduction enhancers forFrontiers in immunology · 2023Article
- Evolution of Gene Therapy, Historical Perspective.Hematology/oncology clinics of North America · 2022Review
- Towards antigen-specific Tregs for type 1 diabetes: Construction and functional assessment of pancreatic endocrine marker, HPi2-based chimeric antigen receptor.Cellular immunology · 2020Article
- Scaffold-Mediated Static Transduction of T Cells for CAR-T Cell Therapy.Advanced healthcare materials · 2020Article
- A Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular Therapies.Scientific reports · 2019Article
- Functional Analysis of an Inducible Promoter Driven by Activation Signals from a Chimeric Antigen Receptor.Molecular therapy oncolytics · 2019Article
- Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with αJournal of virology · 2017Article
- Efficient Transduction of Human and Rhesus Macaque Primary T Cells by a Modified Human Immunodeficiency Virus Type 1-Based Lentiviral Vector.Human gene therapy · 2017Article
- Serial Activation of the Inducible Caspase 9 Safety Switch After Human Stem Cell Transplantation.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Characterization of an Additional Splice Acceptor Site Introduced into CYP4B1 in Hominoidae during Evolution.PloS one · 2015Article
- Identification of amino acid determinants in CYP4B1 for optimal catalytic processing of 4-ipomeanol.The Biochemical journal · 2015Article
- Evaluation of engraftment and immunological tolerance after reduced intensity conditioning in a rhesus hematopoietic stem cell gene therapy model.Gene therapy · 2014Article
- Article
- Peptide nanofibrils boost retroviral gene transfer and provide a rapid means for concentrating viruses.Nature nanotechnology · 2013Article
- In vivo selection of CD4(+) T cells transduced with a gamma-retroviral vector expressing a single-chain intrabody targeting HIV-1 tat.Human gene therapy · 2012Article
- Article
- Antithetical effects of corticosterone and dibutyryl cAMP on adenosine deaminase in the gastrointestinal tract of chicken during postnatal development.Molecular and cellular biochemistry · 2009Article
- Retroviral transduction of murine primary T lymphocytes.Methods in molecular biology (Clifton, N.J.) · 2009Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Primary human T lymphocytes are powerful targets for genetic modification, although the use of these targets in human gene therapy protocols has been hampered by low levels of transduction. We have shown previously that significant increases in the transduction of hematopoietic stem and progenitor cells with retroviral vectors can be obtained by the colocalization of the retrovirus and target cells on specific fibronectin (FN) adhesion domains (H. Hanenberg, X. L. Xiao, D. Dilloo, K. Hashino, I. Kato, and D. A. Williams, Nat. Med. 2:876-882, 1996). We studied the transfer of genes into primary T lymphocytes by using FN-assisted retroviral gene transfer. Activated T lymphocytes were infected for three consecutive days on the recombinant FN fragment CH-296 with a retroviral vector encoding the murine B7-1 protein. Transduced lymphocytes were analyzed for murine B7-1 expression, and it was found that under optimal conditions, 80 to 89% of the CD3+ lymphocytes were transduced. Gene transfer was predominantly augmented by the interaction between VLA-4 on the T lymphocytes and the FN adhesion site CS-1. Adenosine deaminase (ADA)-deficient primary T lymphocytes transduced on CH-296 with a retrovirus encoding murine ADA (mADA) exhibited levels of mADA activity severalfold higher than the levels of the endogenous human ADA protein observed in normal human T lymphocytes. Strikingly, the long-term expression of the transgene was dependent on the activation status of the lymphocytes. This approach will have important applications in human gene therapy protocols targeting primary T lymphocytes.
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