ArticleJournal of bacteriology1998
In vitro and in vivo oxidation of methionine residues in small, acid-soluble spore proteins from Bacillus species.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- New Thoughts on an Old Topic: Secrets of Bacterial Spore Resistance Slowly Being Revealed.Microbiology and molecular biology reviews : MMBR · 2023Review
- Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure.Extremophiles : life under extreme conditions · 2018Article
- The Role of Methionine Sulfoxide Reductases in Oxidative Stress Tolerance and Virulence ofAntioxidants (Basel, Switzerland) · 2018Review
- Virology. A virus that infects a hyperthermophile encapsidates A-form DNA.Science (New York, N.Y.) · 2015Article
- Multiple proteases to localize oxidation sites.PloS one · 2015Article
- Expression of Four Methionine Sulfoxide Reductases in Staphylococcus aureus.International journal of microbiology · 2012Article
- Spx mediates oxidative stress regulation of the methionine sulfoxide reductases operon in Bacillus subtilis.BMC microbiology · 2008Article
- Structure of a protein-DNA complex essential for DNA protection in spores of Bacillus species.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Antisense-RNA-mediated decreased synthesis of small, acid-soluble spore proteins leads to decreased resistance of clostridium perfringens spores to moist heat and UV radiation.Applied and environmental microbiology · 2007Article
- Tricksy business: transcriptome analysis reveals the involvement of thioredoxin A in redox homeostasis, oxidative stress, sulfur metabolism, and cellular differentiation in Bacillus subtilis.Journal of bacteriology · 2005Article
- Inactivation of Bacillus stearothermophilus leucine aminopeptidase II by hydrogen peroxide and site-directed mutagenesis of methionine residues on the enzyme.The protein journal · 2004Article
- Effects of carboxy-terminal modifications and pH on binding of a Bacillus subtilis small, acid-soluble spore protein to DNA.Journal of bacteriology · 2003Article
- E. coli methionine sulfoxide reductase with a truncated N terminus or C terminus, or both, retains the ability to reduce methionine sulfoxide.Protein science : a publication of the Protein Society · 2001Article
- An alpha/beta-type, small, acid-soluble spore protein which has very high affinity for DNA prevents outgrowth of Bacillus subtilis spores.Journal of bacteriology · 2001Article
- Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments.Microbiology and molecular biology reviews : MMBR · 2000Review
- The solid state oxidation of methionine containing peptide: a preliminary study using time of flight secondary ion mass spectrometry.Pharmaceutical research · 2000Article
- Differential regulation of plastidial and cytosolic isoforms of peptide methionine sulfoxide reductase in Arabidopsis.Plant physiology · 2000Article
- Oxidative stress in microorganisms--I. Microbial vs. higher cells--damage and defenses in relation to cell aging and death.Folia microbiologica · 1999Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Methionine residues in alpha/beta-type small, acid-soluble spore proteins (SASP) of Bacillus species were readily oxidized to methionine sulfoxide in vitro by t-butyl hydroperoxide (tBHP) or hydrogen peroxide (H2O2). These oxidized alpha/beta-type SASP no longer bound to DNA effectively, but DNA binding protected alpha/beta-type SASP against methionine oxidation by peroxides in vitro. Incubation of an oxidized alpha/beta-type SASP with peptidyl methionine sulfoxide reductase (MsrA), which can reduce methionine sulfoxide residues back to methionine, restored the alpha/beta-type SASP's ability to bind to DNA. Both tBHP and H2O2 caused some oxidation of the two methionine residues of an alpha/beta-type SASP (SspC) in spores of Bacillus subtilis, although one methionine which is highly conserved in alpha/beta-type SASP was only oxidized to a small degree. However, much more methionine sulfoxide was generated by peroxide treatment of spores carrying a mutant form of SspC which has a lower affinity for DNA. MsrA activity was present in wild-type B. subtilis spores. However, msrA mutant spores were no more sensitive to H2O2 than were wild-type spores. The major mechanism operating for dealing with oxidative damage to alpha/beta-type SASP in spores is DNA binding, which protects the protein's methionine residues from oxidation both in vitro and in vivo. This may be important in vivo since alpha/beta-type SASP containing oxidized methionine residues no longer bind DNA well and alpha/beta-type SASP-DNA binding is essential for long-term spore survival.
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Registered trials
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