ArticleInfection and immunity1998
The Legionella pneumophila icmGCDJBF genes are required for killing of human macrophages.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 111 citations in OpenAlex.
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Modulation of host cell pathways bymLife · 2025Review
- The Legionella pneumophila Dot/Icm Type IV Secretion System is Structurally and Functionally Resilient in Absence of Species-specific Proteins Dis2 and Dis3.Journal of molecular biology · 2025Article
- Legionella pneumophila Risk from Cooling Tower Systems in China.Applied and environmental microbiology · 2022Article
- Structural analysis of theeLife · 2020Article
- Type VI Secretion System in PathogenicFrontiers in microbiology · 2019Review
- Population structure and minimum core genome typing of Legionella pneumophila.Scientific reports · 2016Article
- Native structure of a type IV secretion system core complex essential for Legionella pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- A Type VI secretion system encoding locus is required for Bordetella bronchiseptica immunomodulation and persistence in vivo.PloS one · 2012Article
- Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain.Microbiology (Reading, England) · 2011Article
- Dot/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages.mBio · 2011Article
- Type IVB Secretion Systems of Legionella and Other Gram-Negative Bacteria.Frontiers in microbiology · 2011Article
- Molecular Characterization of Exploitation of the Polyubiquitination and Farnesylation Machineries of Dictyostelium Discoideum by the AnkB F-Box Effector of Legionella Pneumophila.Frontiers in microbiology · 2011Article
- Molecular pathogenesis of infections caused by Legionella pneumophila.Clinical microbiology reviews · 2010Review
- Environmental mimics and the Lvh type IVA secretion system contribute to virulence-related phenotypes of Legionella pneumophila.Infection and immunity · 2007Article
- How to become a uropathogen: comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Characterization of Legionella pneumophila pmiA, a gene essential for infectivity of protozoa and macrophages.Infection and immunity · 2005Article
- sciS, an icmF homolog in Salmonella enterica serovar Typhimurium, limits intracellular replication and decreases virulence.Infection and immunity · 2005Article
- The DotL protein, a member of the TraG-coupling protein family, is essential for Viability of Legionella pneumophila strain Lp02.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previously, a collection of mutants of Legionella pneumophila that had lost the ability to multiply within and kill human macrophages was generated by Tn903dIIlacZ transposon mutagenesis and classified into DNA hybridization groups. A subset of these mutants was complemented by a plasmid, pMW100, containing a 13.5-kb genomic DNA insert. This plasmid restored the ability to multiply within and produce cytopathic effects on human macrophages to members of DNA hybridization groups II, IV, VI, and XVII. A region of the genomic insert of pMW100 was sequenced, and eight potential genes were identified and named icmE, icmG, icmC, icmD, icmJ, icmB, icmF, and tphA. None of the genes encode potential protein products with significant homology to previously characterized proteins, except for tphA, whose product has significant homology to a family of metabolite/H+ symport proteins from gram-negative bacteria. The positions of the Tn903dIIlacZ insertions within the genes were determined by nucleotide sequencing. No Tn903dIIlacZ insertions mapped to icmG, icmJ, or tphA; therefore, these loci were mutated to test whether they were required for macrophage killing. Complementation analysis was used to evaluate the roles of the potential gene products and provide information on the organization of transcriptional units within the region. The results indicate that all identified open reading frames except tphA are required for killing of human macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.