ArticleGenes & development1998
The yeast Ada complex mediates the ligand-dependent activation function AF-2 of retinoid X and estrogen receptors.
Article in Genes & development, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 65 citations in OpenAlex.
- Step-brothers in arms, SAGA and ATAC co-activator complexes, use different strategies.The EMBO journal · 2026Review
- The nuclear receptor 4A family members: mediators in human disease and autophagy.Cellular & molecular biology letters · 2020Review
- Tale of a multifaceted co-activator, hADA3: from embryogenesis to cancer and beyond.Open biology · 2016Review
- Altered nuclear cofactor switching in retinoic-resistant variants of the PML-RARα oncoprotein of acute promyelocytic leukemia.Proteins · 2012Article
- Human ADA3 regulates RARalpha transcriptional activity through direct contact between LxxLL motifs and the receptor coactivator pocket.Nucleic acids research · 2010Article
- Identification of a small TAF complex and its role in the assembly of TAF-containing complexes.PloS one · 2007Article
- T:G mismatch-specific thymine-DNA glycosylase (TDG) as a coregulator of transcription interacts with SRC1 family members through a novel tyrosine repeat motif.Nucleic acids research · 2005Article
- Two different Drosophila ADA2 homologues are present in distinct GCN5 histone acetyltransferase-containing complexes.Molecular and cellular biology · 2003Article
- Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3.Molecular and cellular biology · 2002Article
- ADA3-containing complexes associate with estrogen receptor alpha.Nucleic acids research · 2002Article
- Distinct mutations in yeast TAF(II)25 differentially affect the composition of TFIID and SAGA complexes as well as global gene expression patterns.Molecular and cellular biology · 2002Article
- Transcriptional repression by neuron-restrictive silencer factor is mediated via the Sin3-histone deacetylase complex.Molecular and cellular biology · 2000Article
- The essential cofactor TRRAP recruits the histone acetyltransferase hGCN5 to c-Myc.Molecular and cellular biology · 2000Article
- The human TFIID components TAF(II)135 and TAF(II)20 and the yeast SAGA components ADA1 and TAF(II)68 heterodimerize to form histone-like pairs.Molecular and cellular biology · 2000Article
- Histone acetyltransferase complexes can mediate transcriptional activation by the major glucocorticoid receptor activation domain.Molecular and cellular biology · 1999Article
- P/CAF associates with cyclin D1 and potentiates its activation of the estrogen receptor.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Role of the essential yeast protein PSU1 in p6anscriptional enhancement by the ligand-dependent activation function AF-2 of nuclear receptors.The EMBO journal · 1999Article
- Unbinding of retinoic acid from its receptor studied by steered molecular dynamics.Biophysical journal · 1999Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear receptors can function as ligand-inducible transregulators in both mammalian and yeast cells, indicating that important features of control of transcription have been conserved throughout evolution. Here, we report the isolation and characterization of a yeast protein that exhibits properties expected for a coactivator/mediator of the ligand-dependent activation function AF-2 present in the ligand-binding domain (LBD, region E) of the retinoid X (RXRalpha) and estrogen (ERalpha) receptors. This protein is identical to Ada3, a component of the yeast Ada coactivator complex. We demonstrate that: (1) the region encompassing residues 347-702 of Ada3 interacts with the LBD of RXRalpha and ERalpha in a ligand-dependent manner in yeast; (2) this interaction corresponds to a direct binding and requires the integrity of the core of the AF-2 activating domain (AF-2 AD) of both RXRalpha and ERalpha; (3) Ada3 as well as Ada2 and Gcn5, two other components of the Ada complex, are required for maximal AF-2 activity in yeast; and (4) Ada3 is able to enhance the AF-2 activity of RXRalpha and ERalpha when overexpressed in yeast and mammalian cells. Taken together, these data indicate that ligand-dependent transactivation by RXRalpha and ERalpha in yeast is mediated at least in part by the Ada complex, in which the Ada3 subunit directly binds to the holoreceptor LBD.
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