ArticleApplied and environmental microbiology1998
Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.1 and action of the gene product on kraft pulp.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 104 citations in OpenAlex.
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- Insights on Microbial Communities Inhabiting Non-Volcanic Hot Springs.International journal of molecular sciences · 2022Article
- High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca.Scientific reports · 2020Article
- Solid-binding peptides for immobilisation of thermostable enzymes to hydrolyse biomass polysaccharides.Biotechnology for biofuels · 2017Article
- The Complete Genome Sequence of HyperthermophileFrontiers in microbiology · 2016Article
- Distinct roles for carbohydrate-binding modules of glycoside hydrolase 10 (GH10) and GH11 xylanases from Caldicellulosiruptor sp. strain F32 in thermostability and catalytic efficiency.Applied and environmental microbiology · 2015Article
- Phospholipase Cγ1 connects the cell membrane pathway to the nuclear receptor pathway in insect steroid hormone signaling.The Journal of biological chemistry · 2014Article
- Improvement of alkali stability and thermostability of Paenibacillus campinasensis Family-11 xylanase by directed evolution and site-directed mutagenesis.Journal of industrial microbiology & biotechnology · 2014Article
- Identification and characterization of novel cellulolytic and hemicellulolytic genes and enzymes derived from German grassland soil metagenomes.Biotechnology letters · 2012Article
- Selective advantage of resistant strains at trace levels of antibiotics: a simple and ultrasensitive color test for detection of antibiotics and genotoxic agents.Antimicrobial agents and chemotherapy · 2011Article
- Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.Applied and environmental microbiology · 2010Article
- Molecular diversity and catalytic activity of Thermus DNA polymerases.Extremophiles : life under extreme conditions · 2009Article
- The Anabaena sensory rhodopsin transducer defines a novel superfamily of prokaryotic small-molecule binding domains.Biology direct · 2009Article
- Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin.Extremophiles : life under extreme conditions · 2009Article
- Increased production of xylanase by expression of a truncated version of the xyn11A gene from Nonomuraea flexuosa in Trichoderma reesei.Applied and environmental microbiology · 2007Article
- Improving the alkalophilic performances of the Xyl1 xylanase from Streptomyces sp. S38: structural comparison and mutational analysis.Protein science : a publication of the Protein Society · 2005Article
- Engineering the thermostability of Trichoderma reesei endo-1,4-beta-xylanase II by combination of disulphide bridges.Extremophiles : life under extreme conditions · 2004Article
- Thermophilic bacterial DNA polymerases with reverse-transcriptase activity.Extremophiles : life under extreme conditions · 2004Article
- The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus.Current genetics · 2003Article
- Sequencing and expression of additional xylanase genes from the hyperthermophile Thermotoga maritima FjSS3B.1.Applied and environmental microbiology · 2000Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A two-step PCR protocol was used to identify and sequence a family 11 xylanase gene from Dictyoglomus thermophilum Rt46B.1. Family 11 xylanase consensus fragments (GXCFs) were amplified from Rt46B.1 genomic DNA by using different sets of consensus PCR primers that exhibited broad specificity for conserved motifs within fungal and/or bacterial family 11 xylanase genes. On the basis of the sequences of a representative sample of the GXCFs a single family 11 xylanase gene (xynB) was identified. The entire gene sequence was obtained in the second step by using genomic walking PCR to amplify Rt46B.1 genomic DNA fragments upstream and downstream of the xynB GXCF region. The putative XynB peptide (M(r), 39,800) encoded by the Rt46B.1 xynB open reading frame was a multidomain enzyme comprising an N-terminal catalytic domain (M(r), 22,000) and a possible C-terminal substrate-binding domain (M(r), 13,000) that were separated by a short serine-glycine-rich 23-amino-acid linker peptide. Seven xylanases which differed at their N and C termini were produced from different xynB expression plasmids. All seven xylanases exhibited optimum activity at pH 6.5. However, the temperature optima of the XynB xylanases varied from 70 to 85 degrees C. Pretreatment of Pinus radiata and eucalypt kraft-oxygen pulps with XynB resulted in moderate xylan solubilization and a substantial improvement in the bleachability of these pulps.
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