ArticleApplied and environmental microbiology1998
A flow cytometric method for rapid selection of novel industrial yeast hybrids.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Article
- Crossbreeding of Yeasts Domesticated for Fermentation: Infertility Challenges.International journal of molecular sciences · 2020Review
- Phenotype-Independent Isolation of InterspeciesFrontiers in microbiology · 2019Article
- Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production.Microbial cell factories · 2015Article
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
- Sorting of Streptomyces cell pellets using a complex object parametric analyzer and sorter.Journal of visualized experiments : JoVE · 2014Article
- Applications of flow cytometry in environmental microbiology and biotechnology.Extremophiles : life under extreme conditions · 2009Review
- Epicocconone, a new cell-permeable long Stokes' shift fluorescent stain for live cell imaging and multiplexing.Journal of fluorescence · 2006Article
- Applications of cell sorting in biotechnology.Microbial cell factories · 2006Article
- Generation of a novel Saccharomyces cerevisiae strain that exhibits strong maltose utilization and hyperosmotic resistance using nonrecombinant techniques.Applied and environmental microbiology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We rapidly produced and isolated novel yeast hybrids by using two-color flow cytometric cell sorting. We labeled one parent strain with a fluorescent green stain and the other parent with a fluorescent orange stain, and hybrids were selected based on their dual orange and green fluorescence. When this technique was applied to the production of hybrids by traditional mating procedures, more than 96% of the isolates were hybrids. When it was applied to rare mating, three hybrids were identified among 50 isolates enriched from a population containing 2 x 10(6) cells. This technology is not dependent on genetic markers and has applications in the development of improved industrial yeast strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.