ArticleApplied and environmental microbiology1998
Identification and analysis of genes involved in anaerobic toluene metabolism by strain T1: putative role of a glycine free radical.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 101 citations in OpenAlex.
- Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.Biochemistry · 2026Review
- Article
- Structural basis for anaerobic alkane activation by a multisubunit glycyl radical enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Flocculation-Related Gene Identification by Whole-Genome Sequencing of Thauera aminoaromatica MZ1T Floc-Defective Mutants.Applied and environmental microbiology · 2015Article
- Anaerobic oxidation of long-chain n-alkanes by the hyperthermophilic sulfate-reducing archaeon, Archaeoglobus fulgidus.The ISME journal · 2014Article
- Diversity of benzylsuccinate synthase-like (bssA) genes in hydrocarbon-polluted marine sediments suggests substrate-dependent clustering.Applied and environmental microbiology · 2013Article
- Identification of FeS clusters in the glycyl-radical enzyme benzylsuccinate synthase via EPR and Mössbauer spectroscopy.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2012Article
- Combined genomic and proteomic approaches identify gene clusters involved in anaerobic 2-methylnaphthalene degradation in the sulfate-reducing enrichment culture N47.Journal of bacteriology · 2010Article
- Kinetics for tautomerizations and dissociations of triglycine radical cations.Journal of the American Society for Mass Spectrometry · 2009Article
- Anaerobic catabolism of aromatic compounds: a genetic and genomic view.Microbiology and molecular biology reviews : MMBR · 2009Review
- Anaerobic functionalization of unactivated C-H bonds.Current opinion in chemical biology · 2009Review
- Identification and expression of benzylsuccinate synthase genes in a toluene-degrading methanogenic consortium.Applied and environmental microbiology · 2007Article
- Mechanism of benzylsuccinate synthase probed by substrate and isotope exchange.Journal of the American Chemical Society · 2006Article
- Comparison of mechanisms of alkane metabolism under sulfate-reducing conditions among two bacterial isolates and a bacterial consortium.Applied and environmental microbiology · 2006Article
- Anaerobic degradation of p-Xylene by a sulfate-reducing enrichment culture.Current microbiology · 2005Article
- Substrate-dependent regulation of anaerobic degradation pathways for toluene and ethylbenzene in a denitrifying bacterium, strain EbN1.Journal of bacteriology · 2005Article
- Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1.Applied and environmental microbiology · 2004Article
- Recent advances in petroleum microbiology.Microbiology and molecular biology reviews : MMBR · 2003Review
- Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Anaerobic degradation of ethylbenzene by a new type of marine sulfate-reducing bacterium.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The denitrifying strain T1 is able to grow with toluene serving as its sole carbon source. Two mutants which have defects in this toluene utilization pathway have been characterized. A clone has been isolated, and subclones which contain tutD and tutE, two genes in the T1 toluene metabolic pathway, have been generated. The tutD gene codes for an 864-amino-acid protein with a calculated molecular mass of 97,600 Da. The tutE gene codes for a 375-amino-acid protein with a calculated molecular mass of 41,300 Da. Two additional small open reading frames have been identified, but their role is not known. The TutE protein has homology to pyruvate formate-lyase activating enzymes. The TutD protein has homology to pyruvate formate-lyase enzymes, including a conserved cysteine residue at the active site and a conserved glycine residue that is activated to a free radical in this enzyme. Site-directed mutagenesis of these two conserved amino acids shows that they are also essential for the function of TutD.
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