ArticleMolecular biology of the cell1998
A role for katanin-mediated axonemal severing during Chlamydomonas deflagellation.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 85 citations in OpenAlex.
- Taking Down the Primary Cilium: Pathways for Disassembly in Differentiating Cells.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- CCDC66 regulation of cytoskeleton and cilia stability is important for signaling and epithelial organization.PLoS biology · 2025Article
- Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Cilia and Extracellular Vesicles in Brain Development and Disease.Biological psychiatry · 2024Review
- Review
- Appearing and disappearing acts of cilia.Journal of biosciences · 2023Review
- Combinatorial and antagonistic effects of tubulin glutamylation and glycylation on katanin microtubule severing.Developmental cell · 2022Article
- Shedding of ciliary vesicles at a glance.Journal of cell science · 2022Article
- Review
- The Mammalian Family of Katanin Microtubule-Severing Enzymes.Frontiers in cell and developmental biology · 2021Review
- Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes.Trends in cell biology · 2021Review
- Cilium axoneme internalization and degradation in chytrid fungi.Cytoskeleton (Hoboken, N.J.) · 2020Article
- MKS-NPHP module proteins control ciliary shedding at the transition zone.PLoS biology · 2020Article
- Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.PLoS biology · 2019Article
- Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The regulation of cilium assembly and disassembly in development and disease.Development (Cambridge, England) · 2018Review
- Review
- A Flow Cytometry-Based Approach for the Isolation and Characterization of Neural Stem Cell Primary Cilia.Frontiers in cellular neuroscience · 2018Article
- A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel.G3 (Bethesda, Md.) · 2016Article
- Cilium assembly and disassembly.Nature cell biology · 2016Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Deflagellation of Chlamydomonas reinhardtii, and other flagellated and ciliated cells, is a highly specific process that involves signal-induced severing of the outer doublet microtubules at a precise site in the transition region between the axoneme and basal body. Although the machinery of deflagellation is activated by Ca2+, the mechanism of microtubule severing is unknown. Severing of singlet microtubules has been observed in vitro to be catalyzed by katanin, a heterodimeric adenosine triphosphatase that can remove tubulin subunits from the walls of stable microtubules. We found that purified katanin induced an ATP-dependent severing of the Chlamydomonas axoneme. Using Western blot analysis and indirect immunofluorescence, we demonstrate that Chlamydomonas expresses a protein that is recognized by an anti-human katanin antibody and that this protein is localized, at least in part, to the basal body complex. Using an in vitro severing assay, we show that the protein(s) responsible for Ca2+-activated outer doublet severing purify with the flagellar-basal body complex. Furthermore, deflagellation of purified flagellar-basal body complexes is significantly blocked by the anti-katanin antibody. Taken together, these data suggest that a katanin-like mechanism may mediate the severing of the outer doublet microtubules during Chlamydomonas deflagellation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.