ArticleMolecular biology of the cell1998
Endocytic clathrin-coated pit formation is independent of receptor internalization signal levels.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular thermodynamics of receptor competition for endocytic uptake.Soft matter · 2019Article
- Receptor Heterodimerization Modulates Endocytosis through Collaborative and Competitive Mechanisms.Biophysical journal · 2019Article
- Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation.PLoS pathogens · 2019Article
- Membrane Trafficking in Neuronal Development: Ins and Outs of Neural Connectivity.International review of cell and molecular biology · 2016Article
- Nanomagnetic activation as a way to control the efficacy of nucleic acid delivery.Pharmaceutical research · 2015Article
- Divergent modes for cargo-mediated control of clathrin-coated pit dynamics.Molecular biology of the cell · 2013Article
- Tickets to ride: selecting cargo for clathrin-regulated internalization.Nature reviews. Molecular cell biology · 2009Review
- Premelanosome amyloid-like fibrils are composed of only golgi-processed forms of Pmel17 that have been proteolytically processed in endosomes.The Journal of biological chemistry · 2008Article
- Mint3/X11gamma is an ADP-ribosylation factor-dependent adaptor that regulates the traffic of the Alzheimer's Precursor protein from the trans-Golgi network.Molecular biology of the cell · 2008Article
- G protein-coupled receptor sorting to endosomes and lysosomes.Annual review of pharmacology and toxicology · 2008Review
- The role of cargo proteins in GGA recruitment.Traffic (Copenhagen, Denmark) · 2007Article
- A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors.Molecular biology of the cell · 2006Article
- Clathrin-dependent endocytosis.The Biochemical journal · 2004Review
- G protein-coupled receptor/arrestin3 modulation of the endocytic machinery.The Journal of cell biology · 2002Article
- Rigidity of triskelion arms and clathrin nets.Biophysical journal · 2000Article
- Phosphoinositide-AP-2 interactions required for targeting to plasma membrane clathrin-coated pits.The Journal of cell biology · 1999Article
- Acute cholesterol depletion inhibits clathrin-coated pit budding.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- High-affinity binding of the AP-1 adaptor complex to trans-golgi network membranes devoid of mannose 6-phosphate receptors.Molecular biology of the cell · 1999Article
- Molecular aspects of the endocytic pathway.The Biochemical journal · 1998Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The mechanisms responsible for coated pit formation in cells remain unknown, but indirect evidence has argued both for and against a critical role of receptor cytoplasmic domains in the process. If the endocytic motifs of receptors are responsible for recruiting AP2 to the plasma membrane, thereby driving coated pit formation, then the level of constitutively internalized receptors at the membrane would be expected to govern the steady-state level of coated pits in cells. Here we directly test this hypothesis for broad classes of receptors containing three distinct constitutive internalization signals. Chimeric proteins consisting of an integral membrane reporter protein (Tac) coupled to cytoplasmic domains bearing tyrosine-, di-leucine-, or acidic cluster/casein kinase II-based internalization signals were overexpressed to levels that saturated the internalization pathway. Quantitative confocal immunofluorescence microscopy indicated that the number of plasma membrane clathrin-coated pits and the concentration of their structural components were invariant when comparing cells expressing saturating levels of the chimeric receptors to nonexpressing cells or to cells expressing only the Tac reporter lacking cytoplasmic internalization signals. Biochemical analysis showed that the distribution of coat proteins between assembled coated pits and soluble pools was also not altered by receptor overexpression. Finally, the cellular localizations of AP2 and AP1 were similarly unaffected. These results provide a clear indication that receptor endocytic signals do not determine coated pit levels by directly recruiting AP2 molecules. Rather, the findings support a model in which coated pit formation proceeds through recruitment and activation of AP2, likely through a limited number of regulated docking sites that act independently of endocytic signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.