ArticleMolecular biology of the cell1998
Histone H1 reduces the frequency of initiation in Xenopus egg extract by limiting the assembly of prereplication complexes on sperm chromatin.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.Nature communications · 2020Article
- Emerging roles of linker histones in regulating chromatin structure and function.Nature reviews. Molecular cell biology · 2018Review
- Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication inGenes & development · 2017Article
- Germline-specific H1 variants: the "sexy" linker histones.Chromosoma · 2016Review
- What is the role of histone H1 heterogeneity? A functional model emerges from a 50 year mystery.AIMS biophysics · 2015Article
- Nucleosomes in the neighborhood: new roles for chromatin modifications in replication origin control.Epigenetics · 2011Review
- Control of DNA replication by cyclin-dependent kinases in development.Results and problems in cell differentiation · 2011Review
- Uncoupling of sister replisomes during eukaryotic DNA replication.Molecular cell · 2010Article
- Linker histone phosphorylation regulates global timing of replication origin firing.The Journal of biological chemistry · 2009Article
- Article
- Differential affinity of mammalian histone H1 somatic subtypes for DNA and chromatin.BMC biology · 2007Article
- Article
- Asynchronous replication timing of imprinted loci is independent of DNA methylation, but consistent with differential subnuclear localization.Genes & development · 2003Article
- Nucleoplasmin-mediated chromatin remodelling is required for Xenopus sperm nuclei to become licensed for DNA replication.Nucleic acids research · 2000Article
- DNA replication in quiescent cell nuclei: regulation by the nuclear envelope and chromatin structure.Molecular biology of the cell · 1999Article
- Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Somatic histone H1 reduces both the rate and extent of DNA replication in Xenopus egg extract. We show here that H1 inhibits replication directly by reducing the number of replication forks, but not the rate of fork progression, in Xenopus sperm nuclei. Density substitution experiments demonstrate that those forks that are active in H1 nuclei elongate to form large tracts of fully replicated DNA, indicating that inhibition is due to a reduction in the frequency of initiation and not the rate or extent of elongation. The observation that H1 dramatically reduces the number of replication foci in sperm nuclei supports this view. The establishment of replication competent DNA in egg extract requires the assembly of prereplication complexes (pre-RCs) on sperm chromatin. H1 reduces binding of the pre-RC proteins, XOrc2, XCdc6, and XMcm3, to chromatin. Replication competence can be restored in these nuclei, however, only under conditions that promote the loss of H1 from chromatin and licensing of the DNA. Thus, H1 inhibits replication in egg extract by preventing the assembly of pre-RCs on sperm chromatin, thereby reducing the frequency of initiation. These data raise the interesting possibility that H1 plays a role in regulating replication origin use during Xenopus development.
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