ArticleMolecular biology of the cell1998
Induction of exocytosis from permeabilized mast cells by the guanosine triphosphatases Rac and Cdc42.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 86 citations in OpenAlex.
- Review
- Article
- Molecular Mechanisms of Mast Cell Activation by Cholesterol-Dependent Cytolysins.Frontiers in immunology · 2021Review
- Pathophysiology of bronchoconstriction: role of oxidatively damaged DNA repair.Current opinion in allergy and clinical immunology · 2016Review
- Cytoskeleton in mast cell signaling.Frontiers in immunology · 2012Article
- Article
- Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodeling.American journal of physiology. Cell physiology · 2008Article
- Identification of secernin 1 as a novel immunotherapy target for gastric cancer using the expression profiles of cDNA microarray.Cancer science · 2006Article
- Rho is a presynaptic activator of neurotransmitter release at pre-existing synapses in C. elegans.Genes & development · 2006Article
- Regulated exocytosis in neuroendocrine cells: a role for subplasmalemmal Cdc42/N-WASP-induced actin filaments.Molecular biology of the cell · 2004Article
- Facilitation of Ca(2+)-dependent exocytosis by Rac1-GTPase in bovine chromaffin cells.The Journal of physiology · 2003Article
- Rac GTPase plays an essential role in exocytosis by controlling the fusion competence of release sites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Purification and identification of secernin, a novel cytosolic protein that regulates exocytosis in mast cells.Molecular biology of the cell · 2002Article
- Cdc42p functions at the docking stage of yeast vacuole membrane fusion.The EMBO journal · 2001Article
- Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking.The EMBO journal · 2001Article
- Cdc42-dependent modulation of tight junctions and membrane protein traffic in polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 2001Article
- Vav1 regulates phospholipase cgamma activation and calcium responses in mast cells.Molecular and cellular biology · 2001Article
- Isolation and characterization of effector-loop mutants of CDC42 in yeast.Molecular biology of the cell · 2001Article
- cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network.The EMBO journal · 2001Article
- ATP-dependent membrane assembly of F-actin facilitates membrane fusion.Molecular biology of the cell · 2001Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We applied recombinant forms of the Rho-related small guanosine triphosphatases (GTPases) Rac2 and Cdc42/G25K to permeabilized mast cells to test their ability to regulate exocytotic secretion. Mast cells permeabilized with streptolysin-O leak soluble (cytosol) proteins over a period of 5 min and become refractory to stimulation by Ca2+ and guanosine triphosphate (GTP)gammaS over about 20-30 min. This loss of sensitivity is likely to be due to loss of key regulatory proteins that are normally tethered at intracellular locations. Exogenous proteins that retard this loss of sensitivity to stimulation may be similar, if not identical, to those secretory regulators that are lost. Recombinant Rac and Cdc42/G25K, preactivated by binding GTPgammaS, retard the loss of sensitivity (run-down) and, more importantly, enable secretion to be stimulated by Ca2+ alone. Investigation of the concentration dependence of each of these two GTPases applied individually to the permeabilized cells, and of Cdc42/G25K applied in the presence of an optimal concentration of Rac2, has provided evidence for a shared effector pathway and also a second effector pathway activated by Cdc42/G25K alone. Dominant negative mutant (N17) forms of Rac2 and Cdc42/G25K inhibit secretion induced by Ca2+ and GTPgammaS. Our data suggest that Rac2 and Cdc42 should be considered as candidates for GE, GTPases that mediate exocytosis in cells of hematopoeitic origin.
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