ArticleRNA (New York, N.Y.)1998
Ribosomal release without peptidyl tRNA hydrolysis at translation termination in a eukaryotic system.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Translational buffering by ribosome stalling in upstream open reading frames.PLoS genetics · 2022Article
- The arginine attenuator peptide interferes with the ribosome peptidyl transferase center.Molecular and cellular biology · 2012Article
- Antisense transcription in the human cytomegalovirus transcriptome.Journal of virology · 2007Article
- A nascent polypeptide domain that can regulate translation elongation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Inhibition of translation termination mediated by an interaction of eukaryotic release factor 1 with a nascent peptidyl-tRNA.Molecular and cellular biology · 2002Article
- Abundant early expression of gpUL4 from a human cytomegalovirus mutant lacking a repressive upstream open reading frame.Journal of virology · 2001Article
- The mechanism of tryptophan induction of tryptophanase operon expression: tryptophan inhibits release factor-mediated cleavage of TnaC-peptidyl-tRNA(Pro).Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Nonsense-mediated decay of mRNA for the selenoprotein phospholipid hydroperoxide glutathione peroxidase is detectable in cultured cells but masked or inhibited in rat tissues.Molecular biology of the cell · 2001Article
- Upstream open reading frames as regulators of mRNA translation.Molecular and cellular biology · 2000Review
- Activation of transcription of the human cytomegalovirus early UL4 promoter by the Ets transcription factor binding element.Journal of virology · 2000Article
- Termination and peptide release at the upstream open reading frame are required for downstream translation on synthetic shunt-competent mRNA leaders.Molecular and cellular biology · 2000Article
- Translational control of viral gene expression in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2000Review
- Ribosome shunting in the cauliflower mosaic virus 35S RNA leader is a special case of reinitiation of translation functioning in plant and animal systems.Genes & development · 2000Article
- The evolutionarily conserved eukaryotic arginine attenuator peptide regulates the movement of ribosomes that have translated it.Molecular and cellular biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A 22-codon upstream open reading frame (uORF2) in the human cytomegalovirus UL4 transcript leader inhibits downstream translation in cis. Previous studies revealed that the peptide product of uORF2 mediates this inhibitory effect by interfering with translation termination at its own stop codon. The block at termination results both in accumulation of the nascent uORF2 peptide linked to tRNA(Pro), the tRNA that decodes the final codon of uORF2, and in stalling of ribosomes at the end of uORF2. The stalled ribosomes create a barrier that obstructs ribosomal transit to the downstream cistron. In the current studies, we further investigated the mechanism of uORF2-mediated translational inhibition by assessing the kinetics of uORF2 peptidyl tRNA(Pro) hydrolysis and ribosomal release from the uORF2 termination site. Whereas hydrolysis of a mutant, noninhibitory uORF2 peptidyl tRNA is nearly complete in less than 1 min, hydrolysis of the wild-type peptidyl tRNA is negligible even after 30 min. In spite of this remarkably prolonged block to hydrolysis of the uORF2 peptidyl tRNA(Pro), most ribosomes are released from the uORF2 termination site within 15 min. Thus, peptidyl tRNA hydrolysis is not absolutely required for ribosomal release in this system. These results suggest that a eukaryotic cellular mechanism exists for removing stalled ribosomes from mRNAs in the absence of peptidyl tRNA hydrolysis.
Indexed as
Identifiers
9570317PMC1369606W2109134027What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.