ArticleRNA (New York, N.Y.)1998
Genetic interaction between U6 snRNA and the first intron nucleotide in Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Conservation/Mutation in the Splice Sites of Mitochondrial Solute Carrier Genes of Vertebrates.Journal of molecular evolution · 2016Article
- Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5.Biochemistry · 2014Article
- Structure of the yeast U2/U6 snRNA complex.RNA (New York, N.Y.) · 2012Article
- Metal binding and substrate positioning by evolutionarily invariant U6 sequences in catalytically active protein-free snRNAs.RNA (New York, N.Y.) · 2010Article
- Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.RNA (New York, N.Y.) · 2007Article
- Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing.The EMBO journal · 2006Article
- Ordered partitioning reveals extended splice-site consensus information.Genome research · 2004Article
- Efficient use of a 'dead-end' GA 5' splice site in the human fibroblast growth factor receptor genes.The EMBO journal · 2003Article
- Defining a 5' splice site by functional selection in the presence and absence of U1 snRNA 5' end.RNA (New York, N.Y.) · 2002Article
- Genetic interactions between the 5' and 3' splice site consensus sequences and U6 snRNA during the second catalytic step of pre-mRNA splicing.RNA (New York, N.Y.) · 2001Article
- A ribozyme selected from variants of U6 snRNA promotes 2',5'-branch formation.RNA (New York, N.Y.) · 2001Article
- A tertiary interaction detected in a human U2-U6 snRNA complex assembled in vitro resembles a genetically proven interaction in yeast.RNA (New York, N.Y.) · 2000Article
- Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center.Genes & development · 1999Article
- Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.Genes & development · 1999Article
- Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection.Genes & development · 1999Article
- The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5' splice site.RNA (New York, N.Y.) · 1999Article
- The pre-mRNA 5' cap determines whether U6 small nuclear RNA succeeds U1 small nuclear ribonucleoprotein particle at 5' splice sites.Molecular and cellular biology · 1998Article
- A role for U2/U6 helix Ib in 5' splice site selection.RNA (New York, N.Y.) · 1998Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear pre-mRNA splicing necessitates specific recognition of the pre-mRNA splice sites. It is known that 5' splice site selection requires base pairing of U6 snRNA with intron positions 4-6. However, no factor recognizing the highly conserved 5' splice site GU has yet been identified. We have tested if the known U6 snRNA-pre-mRNA interaction could be extended to include the first intron nucleotides and the conserved 50GAG52 sequence of U6 snRNA. We observe that some combinations of 5' splice site and U6 snRNA mutations produce a specific synthetic block to the first splicing step. In addition, the U6-G52U allele can switch between two competing 5' splice sites harboring different nucleotides following the cleavage site. These results indicate that U6 snRNA position 52 interacts with the first nucleotide of the intron before 5' splice site cleavage. Some combinations of U6 snRNA and pre-mRNA mutations also blocked the second splicing step, suggesting a role for the corresponding nucleotides in a proofreading step before exon ligation. From studies in diverse organisms, various functions have been ascribed to the conserved U6 snRNA 47ACAGAG52 sequence. Our results suggest that these discrepancies might reflect variations between different experimental systems and point to an important conserved role of this sequence in the splicing reaction.
Indexed as
Identifiers
9570316PMC1369605W2160996202What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.