ArticleThe Journal of cell biology1998
Agrin can mediate acetylcholine receptor gene expression in muscle by aggregation of muscle-derived neuregulins.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 116 citations in OpenAlex.
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- Sleep drive is coupled to tissue damage via shedding of Caenorhabditis elegans EGFR ligand SISS-1.Nature communications · 2024Article
- Protein networking: nicotinic acetylcholine receptors and their protein-protein-associations.Molecular and cellular biochemistry · 2024Review
- A link between agrin signalling and CaScientific reports · 2022Article
- Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway.Cell & bioscience · 2021Article
- Emergence of functional neuromuscular junctions in an engineered, multicellular spinal cord-muscle bioactuator.APL bioengineering · 2020Article
- Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle.Neuroscience letters · 2020Review
- Heparin-binding epidermal growth factor-like growth factor promotes neuroblastoma differentiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Recycling of acetylcholine receptors at ectopic postsynaptic clusters induced by exogenous agrin in living rats.Developmental biology · 2014Article
- The tanapoxvirus 15L protein is a virus-encoded neuregulin that promotes viral replication in human endothelial cells.Journal of virology · 2013Article
- Lessons from the embryonic neural stem cell niche for neural lineage differentiation of pluripotent stem cells.Stem cell reviews and reports · 2012Article
- A mouse model of Schwartz-Jampel syndrome reveals myelinating Schwann cell dysfunction with persistent axonal depolarization in vitro and distal peripheral nerve hyperexcitability when perlecan is lacking.The American journal of pathology · 2012Article
- Neuregulin/ErbB regulate neuromuscular junction development by phosphorylation of α-dystrobrevin.The Journal of cell biology · 2011Article
- Role of extracellular matrix proteins and their receptors in the development of the vertebrate neuromuscular junction.Developmental neurobiology · 2011Review
- Matrix metalloproteinase-3 in myasthenia gravis compared to other neurological disorders and healthy controls.Autoimmune diseases · 2011Article
- How does an mRNA find its way? Intracellular localisation of transcripts.Seminars in cell & developmental biology · 2007Review
- Postsynaptic chromatin is under neural control at the neuromuscular junction.The EMBO journal · 2007Article
- Neuromuscular synapse formation in mice lacking motor neuron- and skeletal muscle-derived Neuregulin-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Form and function of developing heart valves: coordination by extracellular matrix and growth factor signaling.Journal of molecular medicine (Berlin, Germany) · 2003Review
- A novel pathway for MuSK to induce key genes in neuromuscular synapse formation.The Journal of cell biology · 2003Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The neural isoforms of agrin can stimulate transcription of the acetylcholine receptor (AChR) epsilon subunit gene in electrically active muscle fibers, as does the motor neuron upon the formation of a neuromuscular junction. It is not clear, however, whether this induction involves neuregulins (NRGs), which stimulate AChR subunit gene transcription in vitro by activating ErbB receptors. In this study, we show that agrin- induced induction of AChR epsilon subunit gene transcription is inhibited in cultured myotubes overexpressing an inactive mutant of the ErbB2 receptor, demonstrating involvement of the NRG/ErbB pathway in agrin- induced AChR expression. Furthermore, salt extracts from the surface of cultured myotubes induce tyrosine phosphorylation of ErbB2 receptors, indicating that muscle cells express biological NRG-like activity on their surface. We further demonstrate by RT-PCR analysis that muscle NRGs have Ig-like domains required for their immobilization at heparan sulfate proteoglycans (HSPGs) of the extracellular matrix. In extrasynaptic regions of innervated muscle fibers in vivo, ectopically expressed neural agrin induces the colocalized accumulation of AChRs, muscle-derived NRGs, and HSPGs. By using overlay and radioligand-binding assays we show that the Ig domain of NRGs bind to the HSPGs agrin and perlecan. These findings show that neural agrin can induce AChR subunit gene transcription by aggregating muscle HSPGs on the muscle fiber surface that then serve as a local sink for focal binding of muscle-derived NRGs to regulate AChR gene expression at the neuromuscular junction.
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