ArticleThe Journal of cell biology1998
Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
65 citing papers in PubMed, 152 citations in OpenAlex.
- Microtubule depolymerization at kinetochores restricts anaphase spindle elongation.Nature chemical biology · 2026Article
- Review
- β-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division.Communications biology · 2026Article
- The cell cycle oscillator and spindle length set the speed of chromosome separation in Drosophila embryos.Current biology : CB · 2025Article
- The cell cycle oscillator and spindle length set the speed of chromosome separation inbioRxiv : the preprint server for biology · 2024Article
- Near millimolar concentration of nucleosomes in mitotic chromosomes from late prometaphase into anaphase.The Journal of cell biology · 2024Article
- Inositol 1, 4, 5-trisphosphate receptor is required for spindle assembly inMolecular biology of the cell · 2022Article
- Attenuated Chromosome Oscillation as a Cause of Chromosomal Instability in Cancer Cells.Cancers · 2021Review
- Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization.Journal of visualized experiments : JoVE · 2021Article
- Active forces shape the metaphase spindle through a mechanical instability.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The power of amphibians to elucidate mechanisms of size control and scaling.Experimental cell research · 2020Review
- Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes.Developmental cell · 2017Article
- Tension sensors reveal how the kinetochore shares its load.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Review
- Fission yeast kinesin-8 controls chromosome congression independently of oscillations.Journal of cell science · 2015Article
- Chromatids segregate without centrosomes during Caenorhabditis elegans mitosis in a Ran- and CLASP-dependent manner.Molecular biology of the cell · 2015Article
- From equator to pole: splitting chromosomes in mitosis and meiosis.Genes & development · 2015Review
- Direct kinetochore-spindle pole connections are not required for chromosome segregation.The Journal of cell biology · 2014Article
- Cell cycle-regulated membrane binding of NuMA contributes to efficient anaphase chromosome separation.Molecular biology of the cell · 2014Article
- Regulation of chromosome speeds in mitosis.Cellular and molecular bioengineering · 2013Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We have used local fluorescence photoactivation to mark the lattice of spindle microtubules during anaphase A in Xenopus extract spindles. We find that both poleward spindle microtubule flux and anaphase A chromosome movement occur at similar rates ( approximately 2 microm/min). This result suggests that poleward microtubule flux, coupled to microtubule depolymerization near the spindle poles, is the predominant mechanism for anaphase A in Xenopus egg extracts. In contrast, in vertebrate somatic cells a "Pacman" kinetochore mechanism, coupled to microtubule depolymerization near the kinetochore, predominates during anaphase A. Consistent with the conclusion from fluorescence photoactivation analysis, both anaphase A chromosome movement and poleward spindle microtubule flux respond similarly to pharmacological perturbations in Xenopus extracts. Furthermore, the pharmacological profile of anaphase A in Xenopus extracts differs from the previously established profile for anaphase A in vertebrate somatic cells. The difference between these profiles is consistent with poleward microtubule flux playing the predominant role in anaphase chromosome movement in Xenopus extracts, but not in vertebrate somatic cells. We discuss the possible biological implications of the existence of two distinct anaphase A mechanisms and their differential contributions to poleward chromosome movement in different cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.