ArticleMolecular and cellular biology1998
Selective disruption of genes transiently induced in differentiating mouse embryonic stem cells by using gene trap mutagenesis and site-specific recombination.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Tracing tumorigenesis in a solid tumor model at single-cell resolution.Nature communications · 2020Article
- Wnt/β-catenin signalling induces MLL to create epigenetic changes in salivary gland tumours.The EMBO journal · 2013Article
- Article
- Bigenic Cre/loxP, puDeltatk conditional genetic ablation.Nucleic acids research · 2004Article
- Inducible gene trapping with drug-selectable markers and Cre/loxP to identify developmentally regulated genes.Molecular and cellular biology · 2004Article
- Genetic interaction between Wnt/beta-catenin and BMP receptor signaling during formation of the AER and the dorsal-ventral axis in the limb.Genes & development · 2003Article
- Disruption of the gene encoding the latent transforming growth factor-beta binding protein 4 (LTBP-4) causes abnormal lung development, cardiomyopathy, and colorectal cancer.Genes & development · 2002Article
- Design of an HIV-1 lentiviral-based gene-trap vector to detect developmentally regulated genes in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genomewide trapping of genes that encode secreted and transmembrane proteins repressed by oncogenic signaling.Genome research · 2001Article
- Gene trapping identifies transiently induced survival genes during programmed cell death.Genome biology · 2001Article
- Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A strategy employing gene trap mutagenesis and site-specific recombination (Cre/loxP) has been used to identify genes that are transiently expressed during early mouse development. Embryonic stem cells expressing a reporter plasmid that codes for neomycin phosphotransferase and Escherichia coli LacZ were infected with a retroviral gene trap vector (U3Cre) carrying coding sequences for Cre recombinase (Cre) in the U3 region. Activation of Cre expression from integrations into active genes resulted in a permanent switching between the two selectable marker genes and consequently the expression of beta-galactosidase (beta-Gal). As a result, clones in which U3Cre had disrupted genes that were only transiently expressed could be selected. Moreover, U3Cre-activating cells acquired a cell autonomous marker that could be traced to cells and tissues of the developing embryo. Thus, when two of the clones with inducible U3Cre integrations were passaged in the germ line, they generated spatial patterns of beta-Gal expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.