ArticleMolecular and cellular biology1998
A promoter region mutation affecting replication of the Tetrahymena ribosomal DNA minichromosome.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins.The EMBO journal · 2009Article
- Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition.The EMBO journal · 2007Article
- A 10 base-pair sequence within Domain III of the GPEET procyclin promoter is essential for the autonomous replication of a plasmid in procyclic Trypanosoma brucei.Molecular and biochemical parasitology · 2007Article
- alpha-tubulin minichromosome promoters in the stichotrichous ciliate Stylonychia lemnae.Eukaryotic cell · 2007Article
- TIF1 Represses rDNA replication initiation, but promotes normal S phase progression and chromosome transmission in Tetrahymena.Molecular biology of the cell · 2005Article
- A paradoxical mutant GATA factor.Eukaryotic cell · 2004Article
- Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification.Molecular and cellular biology · 2003Article
- Differential use of multiple replication origins in the ribosomal DNA episome of the protozoan parasite Entamoeba histolytica.Nucleic acids research · 2003Article
- Interference of the simian virus 40 origin of replication by the cytomegalovirus immediate early gene enhancer: evidence for competition of active regulatory chromatin conformation in a single domain.Molecular and cellular biology · 2000Article
- Three different proteins recognize a multifunctional determinant that controls replication initiation, fork arrest and transcription in Tetrahymena.Nucleic acids research · 2000Article
- Long range cooperative interactions regulate the initiation of replication in the Tetrahymena thermophila rDNA minichromosome.Nucleic acids research · 1999Article
- The one-kilobase DNA fragment upstream of the ardC actin gene of Physarum polycephalum is both a replicator and a promoter.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In the ciliated protozoan Tetrahymena thermophila the ribosomal DNA (rDNA) minichromosome replicates partially under cell cycle control and is also subject to a copy number control mechanism. The relationship between rDNA replication and rRNA gene transcription was investigated by the analysis of replication, transcription, and DNA-protein interactions in a mutant rDNA, the rmm3 rDNA. The rmm3 (for rDNA maturation or maintenance mutant 3) rDNA contains a single-base deletion in the rRNA promoter region, in a phylogenetically conserved sequence element that is repeated in the replication origin region of the rDNA minichromosome. The multicopy rmm3 rDNA minichromosome has a maintenance defect in the presence of a competing rDNA allele in heterozygous cells. No difference in the level of rRNA transcription was found between wild-type and rmm3 strains. However, rmm3 rDNA replicating intermediates exhibited an enhanced pause in the region of the replication origin, roughly 750 bp upstream from the rmm3 mutation. In footprinting of isolated nuclei, the rmm3 rDNA lacked the wild-type dimethyl sulfate (DMS) footprint in the promoter region adjacent to the base change. In addition, a DMS footprint in the origin region was lost in the rmm3 rDNA minichromosome. This is the first reported correlation in this system between an rDNA minichromosome maintenance defect and an altered footprint in the origin region. Our results suggest that a promoter region mutation can affect replication without detectably affecting transcription. We propose a model in which interactions between promoter and origin region complexes facilitate replication and maintenance of the Tetrahymena rDNA minichromosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.