ArticleMolecular and cellular biology1998
Activation of the small GTPase Ral in platelets.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 142 citations in OpenAlex.
- Revisiting RAS family GTPase signaling: effector selectivity and oncogenic bypass.The Biochemical journal · 2026Review
- Characterisation of the Ral GTPase inhibitor RBC8 in human and mouse platelets.Cellular signalling · 2019Article
- Small GTPases in platelet membrane trafficking.Platelets · 2019Review
- The nuts and bolts of the platelet release reaction.Platelets · 2017Review
- The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) Binder Rasa3 Regulates Phosphoinositide 3-kinase (PI3K)-dependent Integrin αIIbβ3 Outside-in Signaling.The Journal of biological chemistry · 2017Article
- Secrets of platelet exocytosis - what do we really know about platelet secretion mechanisms?British journal of haematology · 2013Article
- Elevated RalA activity in the hippocampus of PI3Kγ knock-out mice lacking NMDAR-dependent long-term depression.BMB reports · 2013Article
- The ral exchange factor rgl2 promotes cardiomyocyte survival and inhibits cardiac fibrosis.PloS one · 2013Article
- RalA and RalB proteins are ubiquitinated GTPases, and ubiquitinated RalA increases lipid raft exposure at the plasma membrane.The Journal of biological chemistry · 2012Article
- Control of secondary granule release in neutrophils by Ral GTPase.The Journal of biological chemistry · 2011Article
- Aberrant overexpression of the Rgl2 Ral small GTPase-specific guanine nucleotide exchange factor promotes pancreatic cancer growth through Ral-dependent and Ral-independent mechanisms.The Journal of biological chemistry · 2010Article
- Ral activation promotes melanomagenesis.Oncogene · 2010Article
- Salmonella-directed recruitment of new membrane to invasion foci via the host exocyst complex.Current biology : CB · 2010Article
- Aurora-A phosphorylates, activates, and relocalizes the small GTPase RalA.Molecular and cellular biology · 2010Article
- Protein expression in platelets from six species that differ in their open canalicular system.Platelets · 2010Article
- Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs.The EMBO journal · 2008Article
- Primary platelet signaling cascades and integrin-mediated signaling control ADP-ribosylation factor (Arf) 6-GTP levels during platelet activation and aggregation.The Journal of biological chemistry · 2008Article
- Akt-PDK1 complex mediates epidermal growth factor-induced membrane protrusion through Ral activation.Molecular biology of the cell · 2007Article
- Ral is both necessary and sufficient for the inhibition of myeloid differentiation mediated by Ras.Molecular and cellular biology · 2006Article
- Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ral is a ubiquitously expressed Ras-like small GTPase which is abundantly present in human platelets. The biological function of Ral and the signaling pathway in which Ral is involved are largely unknown. Here we describe a novel method to measure Ral activation utilizing the Ral binding domain of the putative Ral effector RLIP76 as an activation-specific probe. With this assay we investigated the signaling pathway that leads to Ral activation in human platelets. We found that Ral is rapidly activated after stimulation with various platelet agonists, including alpha-thrombin. In contrast, the platelet antagonist prostaglandin I2 inhibited alpha-thrombin-induced Ral activation. Activation of Ral by alpha-thrombin could be inhibited by depletion of intracellular Ca2+, whereas the induction of intracellular Ca2+ resulted in the activation of Ral. Our results show that Ral can be activated by extracellular stimuli. Furthermore, we show that increased levels of intracellular Ca2+ are sufficient for Ral activation in platelets. This activation mechanism correlates with the activation mechanism of the small GTPase Rap1, a putative upstream regulator of Ral guanine nucleotide exchange factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.