ArticlePharmaceutical research1998
Possible role of anion exchanger AE2 as the intestinal monocarboxylic acid/anion antiporter.
Article in Pharmaceutical research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- CD38 produces nicotinic acid adenosine dinucleotide phosphate in the lysosome.The Journal of biological chemistry · 2018Article
- Capturing intracellular pH dynamics by coupling its molecular mechanisms within a fully tractable mathematical model.PloS one · 2014Article
- Retinal transfer of nicotinate by H+ -monocarboxylate transporter at the inner blood-retinal barrier.Microvascular research · 2011Article
- Identifying an uptake mechanism for the antiepileptic and bipolar disorder treatment valproic acid using the simple biomedical model Dictyostelium.Journal of cell science · 2011Article
- Kinetic analysis of butyrate transport in human colon adenocarcinoma cells reveals two different carrier-mediated mechanisms.The Biochemical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of the present study was to investigate the transport of organic monocarboxylic acids mediated by the anion exchanger AE2, which has been already reported to be present at several tissue cell membranes, including intestinal brush border membrane in rabbit.
methodsMembrane transport of organic monocarboxylic acids by AE2 was investigated by transient AE2-gene expression in HEK 293 cells and subsequent uptake studies by the cells.
resultsFunctional transfection of AE2 was confirmed by the enhanced 36Cl- efflux from the cells. When preloaded with chloride anion. AE2-transfected cells demonstrated a significantly enhanced [14C]benzoic acid transport activity compared with mock-transfected cells. The AE2-mediated uptake was saturable with kinetic parameters of Km = 0.26 +/- 0.08 mM and Vmax = 6.14 +/- 0.52 nmol/mg protein/3 min and the uptake of [14C]benzoic acid was pH-dependent with a maximal uptake at pH 6.5. AE2-mediated [14C]benzoic acid uptake was inhibited by Cl-, HCO3-, and DIDS. AE2-transfected cells demonstrated significantly enhanced transport activity for nicotinic acid, propionic acid, butyric acid, and valproic acid as well as benzoic acid compared with mock-transfected cells.
conclusionsAE2 is functionally involved in the anion antiport for organic monocarboxylic acids as well as inorganic anions and is supposed to play a partial role in the intestinal transport of organic acids.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.