ArticleMolecular and cellular biochemistry1998
Stability of the contractile assembly and Ca2+-activated tension in adenovirus infected adult cardiac myocytes.
Article in Molecular and cellular biochemistry, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- A Novel Method of Isolating Myofibrils From Primary Cardiomyocyte Culture Suitable for Myofibril Mechanical Study.Frontiers in cardiovascular medicine · 2019Article
- TnI Structural Interface with the N-Terminal Lobe of TnC as a Determinant of Cardiac Contractility.Biophysical journal · 2018Article
- A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart.Circulation research · 2016Article
- Gene transfer into cardiac myocytes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Differential effects of S100 proteins A2 and A6 on cardiac Ca(2+) cycling and contractile performance.Journal of molecular and cellular cardiology · 2014Article
- Molecular determinants of cardiac myocyte performance as conferred by isoform-specific TnI residues.Biophysical journal · 2014Article
- PKCβII modulation of myocyte contractile performance.Journal of molecular and cellular cardiology · 2012Article
- Article
- Molecular dissection of the inward rectifier potassium current (IK1) in rabbit cardiomyocytes: evidence for heteromeric co-assembly of Kir2.1 and Kir2.2.The Journal of physiology · 2003Article
- N-Cadherin: structure, function and importance in the formation of new intercalated disc-like cell contacts in cardiomyocytes.Heart failure reviews · 2000Review
- A nemaline myopathy mutation in alpha-tropomyosin causes defective regulation of striated muscle force production.The Journal of clinical investigation · 1999Article
- Thin filament protein dynamics in fully differentiated adult cardiac myocytes: toward a model of sarcomere maintenance.The Journal of cell biology · 1999Article
- Identification of a contractile deficit in adult cardiac myocytes expressing hypertrophic cardiomyopathy-associated mutant troponin T proteins.The Journal of clinical investigation · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Adenovirus-mediated gene transfer into adult cardiac myocytes in primary culture is a potentially useful method to study the structure and function of the contractile apparatus. However, the consequences of adenovirus infection on the highly differentiated state of the cultured myocyte have not been determined. We report here a detailed analysis of myofilament structure and function over time in primary culture and after adenovirus infection. Adult rat ventricular myocytes in primary culture were infected with a recombinant adenovirus vector expressing either the LacZ or alkaline phosphatase reporter gene. Control and infected myocytes were collected at days 0-7 post-isolation/infection, and myofilament isoform expression was determined by SDS-PAGE and Western blot. Laser scanning densitometry showed that the alpha- to beta-myosin heavy chain ratio, the stoichiometry of the myosin light chains and the expression of the adult troponin T isoform did not change over time in culture or with adenovirus treatment. Importantly, examination of Ca2+-activated tension in single myocytes showed no change in the shape or position of the tension-pCa relationship in the control and adenovirus infected myocytes during primary culture. These results indicate that the structure and function of adult cardiac myocytes are stable in short term primary culture and are not affected by adenovirus infection per se, and therefore provide the foundation for the use of adenovirus-mediated myofilament gene transfer to study contractile apparatus structure and function in adult cardiac myocytes.
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