ArticleProceedings of the National Academy of Sciences of the United States of America1998
Transposition of the mariner element from Drosophila mauritiana in zebrafish.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 124 citations in OpenAlex.
- Transposons As Tools for Functional Genomics in Vertebrate Models.Trends in genetics : TIG · 2017Review
- Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection.Nucleic acids research · 2017Article
- Characterization of irritans mariner-like elements in the olive fruit fly Bactrocera oleae (Diptera: Tephritidae): evolutionary implications.Die Naturwissenschaften · 2016Article
- Efficient transgenesis mediated by pigmentation rescue in zebrafish.BioTechniques · 2016Article
- Forced evolutionInformation sciences · 2015Article
- Evolutionary Design of Gene Networks: Forced Evolution by Genomic Parasites.Parallel processing letters · 2014Article
- A rice Stowaway MITE for gene transfer in yeast.PloS one · 2013Article
- Article
- Transposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification.BMC genomics · 2012Article
- Transposon-mediated BAC transgenesis in zebrafish.Nature protocols · 2011Article
- Article
- Site-directed integration of transgenes: transposons revisited using DNA-binding-domain technologies.Genetica · 2010Article
- Article
- Review
- Analysis of the retina in the zebrafish model.Methods in cell biology · 2010Article
- Physical properties of DNA components affecting the transposition efficiency of the mariner Mos1 element.Molecular genetics and genomics : MGG · 2009Article
- Mariner transposons as genetic tools in vertebrate cells.Genetica · 2009Review
- Profile of Daniel L. Hartl.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.Genome biology · 2007Review
- Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis.Genome biology · 2007Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
With the increased popularity of zebrafish (Danio rerio) for mutagenesis studies, efficient methods for manipulation of its genome are needed. One approach is the use of a transposable element as a vector for gene transfer in this species. We report here the transformation of zebrafish and germ-line transmission of the mariner element from Drosophila mauritiana. The mariner element was selected because its transposition is independent of host-specific factors. One- to two-cell-stage zebrafish embryos were coinjected with a supercoiled plasmid carrying the nonautonomous mariner element peach and mRNA encoding the transposase. Surviving larvae were reared to adulthood, and the transmission of peach to the F1 generation was tested by PCR. Four of the 12 founders, following plasmid injections on 2 different days, transmitted the element to their progeny. Inheritance of the transgene from the F1 to the F2 generation showed a Mendelian pattern. No plasmid sequences were detected by PCR or Southern blot analysis, indicating transposition of peach rather than random integration of the plasmid DNA. These data provide evidence of transformation of a vertebrate with a transposable element and support the host-independent mechanism for transposition of the mariner element. We suggest this system could be used for insertional mutagenesis or for identifying active regions of the genome in the zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.