ArticleProceedings of the National Academy of Sciences of the United States of America1998
Cellular memory: neutrophil orientation reverses during temporally decreasing chemoattractant concentrations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 67 citations in OpenAlex.
- Many Cells Make Light Work: Self-Generated Gradients OrganizeCold Spring Harbor perspectives in biology · 2026Review
- Traveling-wave chemotaxis of neutrophil-like HL-60 cells.Molecular biology of the cell · 2025Article
- Chemokine receptor distribution on the surface of repolarizing T cells.Biophysical journal · 2024Article
- Local negative feedback of Rac activity at the leading edge underlies a pilot pseudopod-like program for amoeboid cell guidance.PLoS biology · 2023Article
- A two-step search and run response to gradients shapes leukocyte navigation in vivo.The Journal of cell biology · 2022Article
- Article
- Four different mechanisms for switching cell polarity.PLoS computational biology · 2021Article
- Progressive mechanical confinement of chemotactic neutrophils induces arrest, oscillations, and retrotaxis.Journal of leukocyte biology · 2018Article
- A dual-docking microfluidic cell migration assay (DIntegrative biology : quantitative biosciences from nano to macro · 2017Article
- Leading from the Back: The Role of the Uropod in Neutrophil Polarization and Migration.Developmental cell · 2016Review
- A neutrophil treadmill to decouple spatial and temporal signals during chemotaxis.Lab on a chip · 2015Article
- Elements of the cellular metabolic structure.Frontiers in molecular biosciences · 2015Article
- Rectified directional sensing in long-range cell migration.Nature communications · 2014Article
- Cellular memory in eukaryotic chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Memory improves precision of cell sensing in fluctuating environments.Scientific reports · 2014Article
- Attractor metabolic networks.PloS one · 2013Article
- Linear conversion of pressure into concentration, rapid switching of concentration, and generation of linear ramps of concentration in a microfluidic device.Biomicrofluidics · 2012Article
- Modeling the NF-κB mediated inflammatory response predicts cytokine waves in tissue.BMC systems biology · 2011Article
- Microfluidic technologies for temporal perturbations of chemotaxis.Annual review of biomedical engineering · 2010Review
- Selective and tunable gradient device for cell culture and chemotaxis study.Lab on a chip · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cell directional orientation or shape polarization is the first cellular step in neutrophil locomotion. To better understand how chemoattractants interact with cells, we studied neutrophil polarization (or shape changes) during exposure to a temporally decreasing chemoattractant signal of N-formyl-methionyl-leucyl-phenylalanine (FMLP) in the absence of a spatial concentration gradient. To accomplish this objective, we used a manifold of differing FMLP concentrations attached to a stopped-flow microscope chamber. Spatial gradients of a fluorescent chemotactic peptide could not be detected in the chamber by using microfluorometry. When FMLP was injected at continually increasing concentrations at 10-s intervals, the shape and relative direction of the neutrophil persisted. However, when temporally decreasing FMLP concentrations were injected, approximately 80% of the cells changed their direction with 44% of the total cells swinging about to 180 degrees +/- 15 degrees. Most of these directional changes involved dissolution of both the lamellipodium and uropod and reformation of these structures 180 degrees from their original positions. This research suggests that neutrophils reverse their morphological polarity when exposed to temporally decreasing ligand concentrations by "remembering" their ligand exposure history and relative direction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.