ArticleProceedings of the National Academy of Sciences of the United States of America1998
A distinct cyclin-dependent kinase-activating kinase of Arabidopsis thaliana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 101 citations in OpenAlex.
- Cdk activation by phosphorylation: linking growth signals to cell cycle control.Biochemical Society transactions · 2025Review
- Study on the interaction preference between CYCD subclass and CDK family members at the poplar genome level.Scientific reports · 2022Article
- Review
- Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes.The Plant journal : for cell and molecular biology · 2019Article
- Genome-wide annotation, expression profiling, and protein interaction studies of the core cell-cycle genes in Phalaenopsis aphrodite.Plant molecular biology · 2014Article
- Identification of a drought-induced rice gene, OsSAP, that suppresses Bax-induced cell death in yeast.Molecular biology reports · 2013Article
- CDKF;1 and CDKD protein kinases regulate phosphorylation of serine residues in the C-terminal domain of Arabidopsis RNA polymerase II.The Plant cell · 2012Article
- Two Arabidopsis cyclin A3s possess G1 cyclin-like features.Plant cell reports · 2010Article
- A rice kinase-protein interaction map.Plant physiology · 2009Article
- Genome-wide identification and expression analysis of rice cell cycle genes.Plant molecular biology · 2007Article
- The Arabidopsis D-type cyclin CYCD4 controls cell division in the stomatal lineage of the hypocotyl epidermis.The Plant cell · 2007Article
- Potential roles for autophosphorylation, kinase activity, and abundance of a CDK-activating kinase (Ee;CDKF;1) during growth in leafy spurge.Plant molecular biology · 2007Article
- A distinct type of cyclin D, CYCD4;2, involved in the activation of cell division in Arabidopsis.Plant cell reports · 2006Article
- Glutamyl-tRNA mediates a switch in RNA polymerase use during chloroplast biogenesis.EMBO reports · 2005Article
- Genome-wide annotation and expression profiling of cell cycle regulatory genes in Chlamydomonas reinhardtii.Plant physiology · 2005Article
- The plant-specific kinase CDKF;1 is involved in activating phosphorylation of cyclin-dependent kinase-activating kinases in Arabidopsis.The Plant cell · 2004Article
- Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Arabidopsis kinome: after the casting.Functional & integrative genomics · 2004Review
- Mechanism targeted discovery of antitumor marine natural products.Current medicinal chemistry · 2004Review
- The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activation of cyclin-dependent kinases (CDKs) requires phosphorylation of a threonine residue within the T-loop catalyzed by CDK-activating kinases (CAKs). Thus far no functional CAK homologue has been reported in plants. We screened an Arabidopsis cDNA expression library for complementation of a budding yeast CAK mutant. A cDNA, cak1At, was isolated that suppressed the CAK mutation in budding yeast, and it also complemented a fission yeast CAK mutant. cak1At encodes a protein related to animal CAKs. The CAK similarity was restricted to the conserved kinase domains, leading to classification of Cak1At as a distinct CDK in the phylogenetic tree. Immunoprecipitates with the anti-Cak1At antibody phosphorylated human CDK2 at the threonine residue (T160) within the T-loop and activated its activity to phosphorylate histone H1. Whereas CAKs in animals and fission yeast are involved in regulation of the cell cycle and basal transcription by phosphorylating the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II, Cak1At did not phosphorylate the CTD. An Arabidopsis CTD-kinase isolated separately from Cak1At was shown to interact with the yeast protein p13(suc1), but it had no CDK2-kinase activity. Therefore, the CTD of RNA polymerase II is probably phosphorylated by a Cdc2-related kinase distinct from Cak1At. cak1At is a single-copy gene in Arabidopsis and is highly expressed in proliferating cells of suspension cultures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.