ArticleProceedings of the National Academy of Sciences of the United States of America1998
Acetylation at Lys-92 enhances signaling by the chemotaxis response regulator protein CheY.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Untangling the Complexity of Two-Component Signal Transduction in Bacteria.Microorganisms · 2025Review
- Acetylomics reveals an extensive acetylation diversity withinmicroLife · 2024Article
- I like therefore I can, and I can therefore I like: the role of self-efficacy and affect in active inference of allostasis.Frontiers in neural circuits · 2024Article
- Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571.Microbiology spectrum · 2023Article
- Contribution of NmSystems · 2021Article
- TheMicrobiology (Reading, England) · 2021Article
- Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective.Frontiers in microbiology · 2021Review
- Expression ofMicroorganisms · 2020Article
- Article
- Structure and function of the archaeal response regulator CheY.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation, Function, and Detection of Protein Acetylation in Bacteria.Journal of bacteriology · 2017Review
- Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress.Microbiology and molecular biology reviews : MMBR · 2015Review
- Glycolysis for Microbiome Generation.Microbiology spectrum · 2015Article
- Changes in the Acetylome and Succinylome of Bacillus subtilis in Response to Carbon Source.PloS one · 2015Article
- CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli.Molecular microbiology · 2015Article
- Reversible acetylation regulates acetate and propionate metabolism in Mycobacterium smegmatis.Microbiology (Reading, England) · 2013Article
- Acetylation of the response regulator RcsB controls transcription from a small RNA promoter.Journal of bacteriology · 2013Article
- Differential lysine acetylation profiles of Erwinia amylovora strains revealed by proteomics.Journal of proteomics · 2013Article
- CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY.Molecular microbiology · 2010Article
- The acetate switch.Microbiology and molecular biology reviews : MMBR · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
When Escherichia coli cells lacking all chemotaxis proteins except the response regulator CheY are exposed to acetate, clockwise flagellar rotation results, indicating the acetate stimulus has activated signaling by CheY. Acetate can be converted to acetyl-CoA by either of two different metabolic pathways, which proceed through acetyl phosphate or acetyl-AMP intermediates. In turn, CheY can be covalently modified by either intermediate in vitro, leading to phosphorylation or acetylation, respectively. Either pathway is sufficient to support the CheY-mediated response to acetate in vivo. Whereas phosphorylation of Asp-57 is a recognized mechanism for activation of CheY to stimulate clockwise flagellar rotation, acetylation of CheY is less well characterized. We found evidence for multiple CheY acetylation sites by mass spectrometry and directly identified Lys-92 and Lys-109 as acetylation sites by Edman degradation of peptides from [14C]acetate-labeled CheY. Replacement of CheY Lys-92, the preferred acetylation site, with Arg has little effect on chemotaxis but completely prevents the response to acetate via the acetyl-AMP pathway. Thus acetylation of Lys-92 activates clockwise signaling by CheY in vivo. The mechanism by which acetylation activates CheY apparently is not simple charge neutralization, nor does it involve enhanced binding to the FliM flagellar switch protein. Thus acetylation probably affects signal generation by CheY at a step after switch binding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.