ArticleJournal of bioenergetics and biomembranes1997
Oscillating Ca2+-induced channel activity obtained in BLM with a mitochondrial membrane component.
Article in Journal of bioenergetics and biomembranes, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- Review
- Inorganic polyphosphate (polyP) as an activator and structural component of the mitochondrial permeability transition pore.Biochemical Society transactions · 2016Review
- Induction of a non-specific permeability transition in mitochondria from Yarrowia lipolytica and Dipodascus (Endomyces) magnusii yeasts.Journal of bioenergetics and biomembranes · 2009Article
- Ca2+-induced phase separation in the membrane of palmitate-containing liposomes and its possible relation to membrane permeabilization.The Journal of membrane biology · 2007Article
- A large, voltage-dependent channel, isolated from mitochondria by water-free chloroform extraction.Biophysical journal · 2005Article
- Palmitic and stearic acids bind Ca2+ with high affinity and form nonspecific channels in black-lipid membranes. Possible relation to Ca2+-activated mitochondrial pores.Journal of bioenergetics and biomembranes · 2001Article
- Calcium-binding properties of the mitochondrial channel-forming hydrophobic component.Journal of bioenergetics and biomembranes · 2000Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oscillations in ion fluxes and membrane potential may be observed in cells and in mitochondria as well. We obtained Ca2+-induced oscillations in channel activity in black-lipid membranes reconstituted with hydrophobic components extracted from mitochondria. Mitoplasts prepared from purified rat liver mitochondria were extracted with ethanol followed by Folch extraction and further partial purification by silicic acid chromatography. Channel activity was measured in lipid bilayers formed from bovine brain lipids and 10% cardiolipin with addition of the purified fractions. The conductance with 10 mM Ca2+ was 100 pS or its multiples. Ca2+ gradients of 4: 1 induced oscillating channel activity for several hours, with initial open states of 40 s and closed states of 56 s; the open times gradually decreasing to 8.6 s. No channel activity was seen without added fractions. The channel activity was associated with a Ca2+-binding lipid, nonpolar, low-molecular-weight fraction that in gel electrophoresis was not stained with Coomassie Blue and did not contain carbohydrate-staining material. 1H-Nuclear magnetic resonance spectra of the substance showed the presence of aliphatic chains and carbonyls, but the detailed structure remains to be elucidated.
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Registered trials
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