ArticleJournal of virology1998
Forced evolution reveals the importance of short open reading frame A and secondary structure in the cauliflower mosaic virus 35S RNA leader.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Multiple exposures to high concentrations of selenate significantly improve selenate tolerability, red elemental selenium (SeWorld journal of microbiology & biotechnology · 2021Article
- Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond.Frontiers in microbiology · 2018Review
- Review
- Massive production of small RNAs from a non-coding region of Cauliflower mosaic virus in plant defense and viral counter-defense.Nucleic acids research · 2011Article
- Translation of cIAP2 mRNA is mediated exclusively by a stress-modulated ribosome shunt.Molecular and cellular biology · 2008Article
- Article
- Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus.RNA (New York, N.Y.) · 2006Article
- The ribosomal shunt translation strategy of cauliflower mosaic virus has evolved from ancient long terminal repeats.Journal of virology · 2006Article
- Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing.Nucleic acids research · 2006Article
- The cauliflower mosaic virus virion-associated protein is dispensable for viral replication in single cells.Journal of virology · 2002Article
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
- Promoters for pregenomic RNA of banana streak badnavirus are active for transgene expression in monocot and dicot plants.Plant molecular biology · 2001Article
- Ribosome shunt is essential for infectivity of cauliflower mosaic virus.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Termination and peptide release at the upstream open reading frame are required for downstream translation on synthetic shunt-competent mRNA leaders.Molecular and cellular biology · 2000Article
- Ribosome shunting in the cauliflower mosaic virus 35S RNA leader is a special case of reinitiation of translation functioning in plant and animal systems.Genes & development · 2000Article
- Interaction of the cauliflower mosaic virus coat protein with the pregenomic RNA leader.Journal of virology · 2000Article
- Translation by ribosome shunting on adenovirus and hsp70 mRNAs facilitated by complementarity to 18S rRNA.Genes & development · 2000Article
- A stable hairpin preceded by a short open reading frame promotes nonlinear ribosome migration on a synthetic mRNA leader.RNA (New York, N.Y.) · 1999Article
- Human papillomavirus type 18 E1 protein is translated from polycistronic mRNA by a discontinuous scanning mechanism.Journal of virology · 1999Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cauliflower mosaic virus pregenomic 35S RNA begins with a long leader sequence containing an extensive secondary structure and up to nine short open reading frames (sORFs), 2 to 35 codons in length. To test whether any of these sORFs are required for virus viability, their start codons were mutated either individually or in various combinations. The resulting viral mutants were tested for infectivity on mechanically inoculated turnip plants. Viable mutants were passaged several times, and the stability of the introduced mutations was analyzed by PCR amplification and sequencing. Mutations at the 5'-proximal sORF A and in the center of the leader resulted in delayed symptom development and in the appearance of revertants. In the central leader region, the predicted secondary structure, rather than the sORF organization, was restored, while true reversions or second-site substitutions in response to mutations of sORF A restored this sORF. Involvement of sORF A and secondary structure of the leader in the virus replication cycle, and especially in translation of the 35S RNA via ribosome shunting, is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.