ArticleJournal of virology1998
The transmembrane domains of Sindbis virus envelope glycoproteins induce cell death.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
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- Induction of apoptosis in MCF-7 cells by the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus Malaysian strain AF2240.Oncology reports · 2013Article
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- Semliki forest virus-induced endoplasmic reticulum stress accelerates apoptotic death of mammalian cells.Journal of virology · 2010Article
- A structural and functional perspective of alphavirus replication and assembly.Future microbiology · 2009Review
- Induction of apoptosis by paramyxovirus simian virus 5 lacking a small hydrophobic gene.Journal of virology · 2003Article
- Langat flavivirus protease NS3 binds caspase-8 and induces apoptosis.Journal of virology · 2002Article
- Differences in cytokine and chemokine responses during neurological disease induced by polytropic murine retroviruses Map to separate regions of the viral envelope gene.Journal of virology · 2001Article
- Rubella virus replication and links to teratogenicity.Clinical microbiology reviews · 2000Review
- Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide.Journal of virology · 2000Article
- Induction of apoptosis by Sindbis virus occurs at cell entry and does not require virus replication.Journal of virology · 1999Article
- Membrane permeabilization by small hydrophobic nonstructural proteins of Japanese encephalitis virus.Journal of virology · 1999Article
- Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells.Journal of virology · 1999Article
- Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein.Journal of virology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Sindbis virus, the prototype alphavirus, kills cells by inducing apoptosis. To investigate potential mechanisms by which Sindbis virus induces apoptosis, we examined whether specific viral gene products were able to induce cell death. Genes encoding the three structural proteins--capsid, the precursor E1 (6K plus E1), and the precursor E2 (P62 or E3 plus E2)--were cotransfected with a beta-galactosidase reporter plasmid in transient-transfection assays in rat prostate adenocarcinoma AT3 cells. Cell death, as determined by measuring the loss of blue cells, was observed in AT3 cells transfected with 6K plus E1 and with P62 but not in cells transfected with capsid. Deletion mutagenesis of P62 indicated that large regions of the cytoplasmic domain and extracellular domain were not essential for the induction of cell death. However, constructs containing the minimal E3 signal sequence fused to the E2 transmembrane domain and the minimal E3 signal sequence fused to the E1 transmembrane domain induced death as efficiently as full-length P62 and 6K plus E1, whereas no cell death was observed after transfection with a control construct containing the E3 signal sequence linked to the transmembrane domain of murine CD4. These data demonstrate that intracellular expression of the transmembrane domains of the Sindbis virus envelope glycoproteins can kill AT3 cells.
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