ArticleJournal of virology1998
Transgenic mice secreting coronavirus neutralizing antibodies into the milk.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Small Non-coding RNAs Associated with Viral Infectious Diseases of Veterinary Importance: Potential Clinical Applications.Frontiers in veterinary science · 2016Review
- (+)-Catechin inhibition of transmissible gastroenteritis coronavirus in swine testicular cells is involved its antioxidation.Research in veterinary science · 2015Article
- The history of monoclonal antibody development - Progress, remaining challenges and future innovations.Annals of medicine and surgery (2012) · 2014Review
- High-dose dietary zinc oxide mitigates infection with transmissible gastroenteritis virus in piglets.BMC veterinary research · 2014Article
- Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus.Archives of virology · 2013Article
- Use of transgenic animals in biotechnology: prospects and problems.Acta naturae · 2013Article
- Establishment of a transgenic mouse model with liver-specific expression of secretory immunoglobulin D.Science China. Life sciences · 2012Article
- Functional recombinant human anti-HAV antibody expressed in milk of transgenic mice.Transgenic research · 2009Article
- High level expression of a functional human/mouse chimeric anti-CD20 monoclonal antibody in milk of transgenic mice.Transgenic research · 2008Article
- Immune response induced by spike protein from transmissible gastroenteritis coronavirus expressed in mouse mammary cells.Virus research · 2007Article
- An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts.Plant biotechnology journal · 2006Article
- Use of virus vectors for the expression in plants of active full-length and single chain anti-coronavirus antibodies.Biotechnology journal · 2006Article
- Maternally derived recombinant human anti-hantavirus monoclonal antibodies are transferred to mouse offspring during lactation and neutralize virus in vitro.Journal of virology · 2006Article
- Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- On the use of post-transcriptional processing elements in transgenes.Transgenic research · 2004Article
- Neutralization of enteric coronaviruses with Escherichia coli cells expressing single-chain Fv-autotransporter fusions.Journal of virology · 2003Article
- Mammary expression of new genes to combat mastitis.Journal of animal science · 2003Review
- Antibody manufacture in transgenic animals and comparisons with other systems.Current opinion in biotechnology · 2002Review
- Therapeutic applications of monoclonal antibodies.The American journal of the medical sciences · 2002Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ten lines of transgenic mice secreting transmissible gastroenteritis coronavirus (TGEV) neutralizing recombinant monoclonal antibodies (rMAbs) into the milk were generated. The rMAb light- and heavy-chain genes were assembled by fusing the genes encoding the variable modules of the murine MAb 6A.C3, which binds an interspecies conserved coronavirus epitope essential for virus infectivity, and a constant module from a porcine myeloma with the immunoglobulin A (IgA) isotype. The chimeric antibody led to dimer formation in the presence of J chain. The neutralization specific activity of the recombinant antibody produced in transiently or stably transformed cells was 50-fold higher than that of a monomeric rMAb with the IgG1 isotype and an identical binding site. This rMAb had titers of up to 10(4) by radioimmunoassay (RIA) and neutralized virus infectivity up to 10(4)-fold. Of 23 transgenic mice, 17 integrated both light and heavy chains, and at least 10 of them transmitted both genes to the progeny, leading to 100% of animals secreting functional TGEV neutralizing antibody during lactation. Selected mice produced milk with TGEV-specific antibody titers higher than 10(6) as determined by RIA, neutralized virus infectivity by 10(6)-fold, and produced up to 6 mg of antibody per ml. Antibody expression levels were transgene copy number independent and integration site dependent. Comicroinjection of the genomic beta-lactoglobulin gene with rMAb light- and heavy-chain genes led to the generation of transgenic mice carrying the three transgenes. The highest antibody titers were produced by transgenic mice that had integrated the antibody and beta-lactoglobulin genes, although the number of transgenic animals generated does not allow a definitive conclusion on the enhancing effect of beta-lactoglobulin cointegration. This approach may lead to the generation of transgenic animals providing lactogenic immunity to their progeny against enteric pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.