ArticleJournal of bacteriology1998
A period-extender gene, pex, that extends the period of the circadian clock in the cyanobacterium Synechococcus sp. strain PCC 7942.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 86 citations in OpenAlex.
- Environmental and molecular noise buffering by the cyanobacterial clock in individual cells.Nature communications · 2025Article
- The effects of PstR, a PadR family transcriptional regulatory factor, in Plesiomonas shigelloides are revealed by transcriptomics.BMC microbiology · 2024Article
- Discovery of nondiazotrophicProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Minimal tool set for a prokaryotic circadian clock.BMC evolutionary biology · 2017Article
- Giving Time Purpose: The Synechococcus elongatus Clock in a Broader Network Context.Annual review of genetics · 2015Review
- Elucidation of the role of clp protease components in circadian rhythm by genetic deletion and overexpression in cyanobacteria.Journal of bacteriology · 2013Article
- Rhythmic and sustained oscillations in metabolism and gene expression of Cyanothece sp. ATCC 51142 under constant light.Frontiers in microbiology · 2013Article
- Temporal and spatial oscillations in bacteria.Nature reviews. Microbiology · 2011Review
- The itty-bitty time machine genetics of the cyanobacterial circadian clock.Advances in genetics · 2011Review
- Simplicity and complexity in the cyanobacterial circadian clock mechanism.Current opinion in genetics & development · 2010Review
- Circadian clock proteins in prokaryotes: hidden rhythms?Frontiers in microbiology · 2010Article
- Cyanobacterial daily life with Kai-based circadian and diurnal genome-wide transcriptional control in Synechococcus elongatus.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- How a cyanobacterium tells time.Current opinion in microbiology · 2008Review
- The circadian clock-related gene pex regulates a negative cis element in the kaiA promoter region.Journal of bacteriology · 2007Article
- Integrating the circadian oscillator into the life of the cyanobacterial cell.Cold Spring Harbor symposia on quantitative biology · 2007Review
- labA: a novel gene required for negative feedback regulation of the cyanobacterial circadian clock protein KaiC.Genes & development · 2007Article
- A novel mutation in kaiC affects resetting of the cyanobacterial circadian clock.Journal of bacteriology · 2005Article
- LdpA: a component of the circadian clock senses redox state of the cell.The EMBO journal · 2005Article
- Molecular evolution of ldpA, a gene mediating the circadian input signal in cyanobacteria.Journal of molecular evolution · 2005Article
- Origin and evolution of circadian clock genes in prokaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We cloned the pS1K1 plasmid in the process of apparently "complementing" a circadian clock mutant of cyanobacterium Synechococcus sp. strain PCC 7942, SP22, which has a 22-h period (T. Kondo, N. F. Tsinoremas, S. S. Golden, C. H. Johnson, S. Kutsuna, and M. Ishiura, Science 266:1233-1236, 1994). Sequence analysis revealed that SP22 did not have a mutation in the genomic DNA segment carried on pS1K1, and the sp22 mutation was later found in a recently cloned new clock gene, kaiC. Therefore, the period-extender gene pex that was carried on pS1K1 was a suppressor gene for the sp22 mutation. The pex gene encoded a protein of 148 amino acid residues. No meaningful homologs were found in DNA or protein databases including the Synechocystis genome database. The pex gene was transcribed from 129 and 164 bp upstream of the translation initiation codon as 0.6-kb transcripts. The Pex protein was detected as a fusion protein with a molecular mass of 15 kDa by the epitope tag fusion method using a c-Myc epitope tag. Disruption of the pex gene in wild-type cells shortened the period of the rhythms by 1 h, although it did not affect other properties of the rhythms, whereas its overexpression extended the period by 3 h with a concomitant reduction in the amplitude of the rhythms. In various clock mutants examined, overexpression caused arrhythmicity. Thus, Pex is likely to function as a modifier of the circadian clock in Synechococcus.
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